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      Transport Capacity of Median Eminence: in vitro Uptake of 3H-LRF

      ,

      Neuroendocrinology

      S. Karger AG

      Median eminence, LRF, Hypothalamus, Anterior pituitary

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          Abstract

          In vitro, median eminence was found to take up <sup>3</sup>H-LRF rapidly, reach equilibrium in approximately 30 min, and achieve an uptake (30 pg LRF/mg) twice that accumulated by anterior pituitary. Conditions which challenged cellular integrity (boiling, treatment with hypertonic saline, etc.) caused marked increases in uptake. Uptake was temperature-dependent and required oxygen and glucose, but was not noticeably ouabain-sensitive. The dissociation of <sup>3</sup>H-LRF from median eminence occurred rapidly into media containing a 1,000-fold greater concentration of unlabeled LRF. Estradiol and testosterone, but not corticosterone, significantly inhibited uptake. Unlabeled LRF and TRF, but not MIF, provided significant competition for the uptake of <sup>3</sup>H-LRF.

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          Author and article information

          Journal
          NEN
          Neuroendocrinology
          10.1159/issn.0028-3835
          Neuroendocrinology
          S. Karger AG
          0028-3835
          1423-0194
          1974
          1974
          25 March 2008
          : 15
          : 3-4
          : 147-157
          Affiliations
          Department of Anatomy, University of Rochester School of Medicine and Dentistry, Rochester, N.Y.
          Article
          122303 Neuroendocrinology 1974;15:147–157
          10.1159/000122303
          4214377
          © 1974 S. Karger AG, Basel

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          Pages: 11
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