In vitro propagation method for production of morphologically and genetically stable plants of different strawberry cultivars

Abstract

Background

As strawberries are susceptible to somaclonal variation when propagated by tissue culture techniques, it is challenging to obtain the true-to-type plants necessary for continuous production of fruits of stable quality. Therefore, we aimed to develop an in vitro propagation method for the production of true-to-type plants of five different strawberry cultivars from meristems cultured in media containing different concentrations of kinetin (Kn).

Results

For all the cultivars, shoot induction was successful only in the meristems cultured in the medium without Kn and the medium containing 0.5 mg L ⁻¹ Kn. The shoots obtained from explants cultured in media supplemented with 0.5 mg L ⁻¹ Kn exhibited better plant growth parameters than those cultured in media without Kn and were genetically stable when compared with conventionally propagated plants for all the cultivars. Vegetative and sexual characters and fruit quality attributes observed in the plants derived from meristems cultured on 0.5 mg L ⁻¹ Kn and the conventionally propagated plants were not significantly different when grown for three continuous growing seasons under greenhouse conditions.

Conclusion

The culture of meristems in the medium containing 0.5 mg L ⁻¹ Kn is suitable for the efficient propagation of true-to-type plants of different strawberry cultivars and continuous production of fruits with stable quality. Hence, we expect that the method presented in this study will be helpful for the commercial production of true-to-type plants generated in vitro for other strawberry cultivars.

Electronic supplementary material

The online version of this article (10.1186/s13007-019-0421-0) contains supplementary material, which is available to authorized users.