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      Standardized method for in vitro antifungal susceptibility testing of Candida albicans biofilms.

      Antimicrobial Agents and Chemotherapy
      Amphotericin B, pharmacology, Antifungal Agents, Biofilms, drug effects, Candida albicans, Cell Count, Colorimetry, Fluconazole, Humans, Microbial Sensitivity Tests, standards, Reproducibility of Results

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          Abstract

          Candida albicans is implicated in many biomaterial-related infections. Typically, these infections are associated with biofilm formation. Cells in biofilms display phenotypic traits that are dramatically different from those of their free-floating planktonic counterparts and are notoriously resistant to antimicrobial agents. Consequently, biofilm-related infections are inherently difficult to treat and to fully eradicate with normal treatment regimens. Here, we report a rapid and highly reproducible microtiter-based colorimetric assay for the susceptibility testing of fungal biofilms, based on the measurement of metabolic activities of the sessile cells by using a formazan salt reduction assay. The assay was used for in vitro antifungal susceptibility testing of several C. albicans strains grown as biofilms against amphotericin B and fluconazole and the increased resistance of C. albicans biofilms against these antifungal agents was demonstrated. Because of its simplicity, compatibility with a widely available 96-well microplate platform, high throughput, and automation potential, we believe this assay represents a promising tool for the standardization of in vitro antifungal susceptibility testing of fungal biofilms.

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          Author and article information

          Journal
          11502517
          90680
          10.1128/AAC.45.9.2475-2479.2001

          Chemistry
          Amphotericin B,pharmacology,Antifungal Agents,Biofilms,drug effects,Candida albicans,Cell Count,Colorimetry,Fluconazole,Humans,Microbial Sensitivity Tests,standards,Reproducibility of Results

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