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      Effects of pH and Light Exposure on the Riboflavin-Binding Capacity in the Rat Lens

      , ,

      Ophthalmic Research

      S. Karger AG

      pH Light exposure, Dialysis, Riboflavin-binding capacity, Lens

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          Abstract

          To elucidate the binding mode of riboflavin to rat lens protein, we examined the effects of light exposure, different pH (4.0, 7.0 and 9.0) and dialysis on the lenticular riboflavin-binding capacity (LRBC). LRBC increased remarkably by light exposure, irrespective of pH. By dialysis, LRBC of a light-exposed incubation mixture decreased remarkably at pH 9.0, however, at pH 4.0 and 7.0, dialysis did not affect it significantly. Decrease in LRBC in light-exposed dialyzed samples at the higher pH may result from release of dialyzable bound riboflavin from binding protein. These data suggest that the dialyzed bound riboflavin at the higher pH may be responsible for a photosensitization of lens protein.

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1989
          1989
          10 December 2009
          : 21
          : 2
          : 93-96
          Affiliations
          Department of Biochemistry, School of Medicine, Iwate Medical University, Uchimaru, Morioka, Japan
          Article
          266784 Ophthalmic Res 1989;21:93–96
          10.1159/000266784
          2734004
          © 1989 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 4
          Categories
          Original Paper

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