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Abstract
Evidence is presented for hormone-controlled adenosine 3',5'-cyclic monophosphate
(cAMP)-mediated incorporation of 14C-acetate by in vitro pheromone gland preparations
of Heliothis armigera. Basal intracellular cAMP concentrations of in vitro pheromone
glands increase 28-fold in the presence of methanolic brain-complex extracts after
5 min of incubation. This intracellular cAMP increase shows a dose-dependent response.
Studies on isolated pheromone gland preparations show that 8-bromo-cAMP added to the
incubation media stimulates acetate incorporation thereby mimicking the hormonal response.
This effect was duplicated by forskolin and isobutyl-methylxanthine. In addition,
forskolin exhibits a dose-dependent stimulatory effect on the incorporation of 14C-acetate
by such isolated in vitro pheromone glands. High-pressure liquid chromatographic analysis
of the radioactive products, extracted by hexane from pheromone glands and media,
revealed a stimulation by forskolin of a product exhibiting the same retention time
as (Z)-11-hexadecenal, the main pheromone component of H. armigera. In addition, thin-layer
chromatographic analysis revealed co-elution with (Z)-11-hexadecenal on stimulation
by 8-bromo-cAMP.