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      Sugarcane ( Saccharum X officinarum): A Reference Study for the Regulation of Genetically Modified Cultivars in Brazil

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          Abstract

          Global interest in sugarcane has increased significantly in recent years due to its economic impact on sustainable energy production. Sugarcane breeding and better agronomic practices have contributed to a huge increase in sugarcane yield in the last 30 years. Additional increases in sugarcane yield are expected to result from the use of biotechnology tools in the near future. Genetically modified (GM) sugarcane that incorporates genes to increase resistance to biotic and abiotic stresses could play a major role in achieving this goal. However, to bring GM sugarcane to the market, it is necessary to follow a regulatory process that will evaluate the environmental and health impacts of this crop. The regulatory review process is usually accomplished through a comparison of the biology and composition of the GM cultivar and a non-GM counterpart. This review intends to provide information on non-GM sugarcane biology, genetics, breeding, agronomic management, processing, products and byproducts, as well as the current technologies used to develop GM sugarcane, with the aim of assisting regulators in the decision-making process regarding the commercial release of GM sugarcane cultivars.

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          Most cited references11

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          Assessment of risk of insect-resistant transgenic crops to nontarget arthropods.

          An international initiative is developing a scientifically rigorous approach to evaluate the potential risks to nontarget arthropods (NTAs) posed by insect-resistant, genetically modified (IRGM) crops. It adapts the tiered approach to risk assessment that is used internationally within regulatory toxicology and environmental sciences. The approach focuses on the formulation and testing of clearly stated risk hypotheses, making maximum use of available data and using formal decision guidelines to progress between testing stages (or tiers). It is intended to provide guidance to regulatory agencies that are currently developing their own NTA risk assessment guidelines for IRGM crops and to help harmonize regulatory requirements between different countries and different regions of the world.
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            Characterisation of the double genome structure of modern sugarcane cultivars (Saccharum spp.) by molecular cytogenetics.

            Cultivated sugarcane clones (Saccharum spp., 2n=100 to 130) are derived from complex interspecific hybridizations between the species S. officinarum and S. spontaneum. Using comparative genomic DNA in situ hybridization, we demonstrated that it is possible to distinguish the chromosomes contributed by these two species in an interspecific F1 hybrid and a cultivated clone, R570. In the interspecific F1 studied, we observed n + n transmission of the parental chromosomes instead of the peculiar 2n + n transmission usually described in such crosses. Among the chromosomes of cultivar R570 (2n = 107-115) about 10% were identified as originating from S. spontaneum and about 10% were identified as recombinant chromosomes between the two species S. officinarum and S. spontaneum. This demonstrated for the first time the occurrence of recombination between the chromosomes of these two species. The rDNA sites were located by in situ hybridization in these two species and the cultivar R570. This supported different basic chromosome numbers and chromosome structural differences between the two species and provided a first bridge between physical and genetical mapping in sugarcane.
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              The Impact of Sugar Cane–Burning Emissions on the Respiratory System of Children and the Elderly

              We analyzed the influence of emissions from burning sugar cane on the respiratory system during almost 1 year in the city of Piracicaba in southeast Brazil. From April 1997 through March 1998, samples of inhalable particles were collected, separated into fine and coarse particulate mode, and analyzed for black carbon and tracer elements. At the same time, we examined daily records of children ( 64 years of age) admitted to the hospital because of respiratory diseases. Generalized linear models were adopted with natural cubic splines to control for season and linear terms to control for weather. Analyses were carried out for the entire period, as well as for burning and nonburning periods. Additional models were built using three factors obtained from factor analysis instead of particles or tracer elements. Increases of 10.2 μg/m3 in particles ≥ 2.5 μm/m3 aerodynamic diameter (PM2.5) and 42.9 μg/m3 in PM10 were associated with increases of 21.4% [95% confidence interval (CI), 4.3–38.5] and 31.03% (95% CI, 1.25–60.21) in child and elderly respiratory hospital admissions, respectively. When we compared periods, the effects during the burning period were much higher than the effects during nonburning period. Elements generated from sugar cane burning (factor 1) were those most associated with both child and elderly respiratory admissions. Our results show the adverse impact of sugar cane burning emissions on the health of the population, reinforcing the need for public efforts to reduce and eventually eliminate this source of air pollution.
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                Author and article information

                Contributors
                adriana.cheavegatti@alellyx.com.br
                newton_macedo@yahoo.com.br
                sizuo.matsuoka@gmail.com
                fernando.reinach@gmail.com
                eugenio.c.ulian@monsanto.com
                Journal
                Trop Plant Biol
                Tropical Plant Biology
                Springer-Verlag (New York )
                1935-9756
                1935-9764
                22 February 2011
                22 February 2011
                March 2011
                : 4
                : 1
                : 62-89
                Affiliations
                [1 ]CanaVialis/Alellyx S.A., Rua James Clerk Maxwell, 320, 13069-380 Campinas, São Paulo Brasil
                [2 ]Centro de Biologia Molecular e Engenharia Genética, Universidade Estadual de Campinas, 13083-875 Campinas, São Paulo Brasil
                [3 ]Universidade Federal do Paraná, Rua dos Funcionários, 1540, Cabral, 80035-050 Curitiba, Paraná Brasil
                [4 ]Centro de Tecnologia Canavieira, CP 162, 13400-970 Piracicaba, São Paulo Brasil
                [5 ]IAC/APTA - Centro de Cana, Instituto Agronômico de Campinas, Rodovia Antonio Duarte Nogueira, Km 321, CP 206, 14032-800 Ribeirão Preto, São Paulo Brazil
                [6 ]Amyris Crystalsev Biocombustíveis Ltda., Rua James Clerk Maxwell, 315, 13069-380 Campinas, São Paulo Brasil
                [7 ]Amyris Crystalsev Biocombustíveis Ltda., Amyris Inc, 5885 Hollis St, Ste 100, Emeryville, CA 94608 USA
                [8 ]Campus de Jaboticabal, Departamento de Tecnologia, Universidade Estadual Paulista, 14884-900 Jaboticabal, SP Brasil
                [9 ]CENA/USP - Laboratório de Melhoramento de Plantas, CP 96, 13400-970 Piracicaba, São Paulo Brasil
                [10 ]Reserva Ecológica do IBGE, CP 08770, 70312-970 Brasília, Distrito Federal Brazil
                [11 ]Embrapa Recursos Genéticos e Biotecnologia, Parque Estação Biológica, Av. W5 Norte, 70770-900, CP 02372 Brasília, Distrito Federal Brasil
                [12 ]Embrapa Recursos Genéticos e Biotecnologia, Empresa Brasileira de Agropecuária, Av. W5 Norte, 70770-900, CP 02372 Brasília, Distrito Federal Brasil
                [13 ]Embrapa Tabuleiros Costeiros, Unidade de Execucao de Pesquisa, BR 104 Norte, Km 85, 57061-970, CP 2013 Maceió, Alagoas Brasil
                [14 ]Campus de Araras, Universidade Federal de São Carlos, Rodovia Anhanguera, Km 174, CP 153, 13600-970 Araras, São Paulo Brasil
                [15 ]Araujo & Macedo Ltda., Rua Oswaldo Cruz, 205, Jardim Santa Cruz, 13601-252 Araras, São Paulo Brasil
                [16 ]Dow Agroscience, Rodovia Anhanguera, Km 344, Jardinópolis, 14680-000 São Paulo, Brasil
                [17 ]Departamento de Genética, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Avenida Pádua Dias, 11, CP 83, 13400-970 Piracicaba, São Paulo Brasil
                Author notes

                Communicated by: Marcelo C. Dornelas

                Article
                9068
                10.1007/s12042-011-9068-3
                3075403
                21614128
                0512d52a-5736-4bc5-9e19-c42273ccdbc2
                © The Author(s) 2011
                History
                : 6 October 2010
                : 13 January 2011
                Categories
                Article
                Custom metadata
                © Springer Science+Business Media, LLC 2011

                Plant science & Botany
                sugarcane,biofuel,ethanol,biosafety,saccharum
                Plant science & Botany
                sugarcane, biofuel, ethanol, biosafety, saccharum

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