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Abstract
A current challenge of the cellulosic ethanol industry is to improve the resistance
of inhibitors present in biomass hydrolysates. RNA-binding protein gene lsm6 was cloned
from industrial Saccharomyces cerevisiae ZU-E8, which is able to conferment glucose
and xylose, and transformed into ZU-E8 via expression vector pRS426. The positive
transformant ZU-910 with over-expressing lsm6 was identified on the culture plates
using high concentration of acetate and re-screened by fermentation test. Fermentation
by the recombinants was performed in a medium containing 80 g/L xylose and 2 g/L acetic
acid or 20 g/L NH(4)Ac/NaAc. After 96 h shaking-flask fermentation, ZU-910 utilized
90.2% xylose with an ethanol yield of 26.9 g/L, which was 8.5- and 10-fold higher
than ZU-E8. Further, in the corn stover hemicellulosic hydrolysate fermentation, both
the xylose conversion and ethanol production by ZU-910 was larger by 50% and 40% than
ZU-E8. ZU-910 has also enhanced tolerance against furfural and SO(4)(2-).