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Removal of PCR inhibitors by silica membranes: evaluating the Amplicor Mycobacterium tuberculosis kit.

Author(s): V Brade, T Bittner, B Böddinghaus, T Wichelhaus

Publication date: 2001-09-30

Journal: Journal of Clinical Microbiology

Keywords: Culture Media, DNA, Bacterial, isolation & purification, Filtration, methods, Humans, Membranes, Artificial, Mycobacterium tuberculosis, classification, genetics, Polymerase Chain Reaction, Reagent Kits, Diagnostic, Sensitivity and Specificity, Silicon Dioxide, Tuberculosis, microbiology

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Abstract

The effectiveness of PCR inhibitor removal by silica membranes in combination with the Amplicor Mycobacterium tuberculosis kit was analyzed for 655 respiratory and nonrespiratory specimens. The overall inhibition rate was reduced from 12.5%, when applying the Amplicor kit alone, to 1.1% with the addition of silica membrane DNA purification.

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PubMed ID:: 11574609

PMC ID:: 88425

DOI:: 10.1128/JCM.39.10.3750-3752.2001

ScienceOpen disciplines: Chemistry

Keywords: Culture Media,DNA, Bacterial,isolation & purification,Filtration,methods,Humans,Membranes, Artificial,Mycobacterium tuberculosis,classification,genetics,Polymerase Chain Reaction,Reagent Kits, Diagnostic,Sensitivity and Specificity,Silicon Dioxide,Tuberculosis,microbiology

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ScienceOpen disciplines: Chemistry

Removal of PCR inhibitors by silica membranes: evaluating the Amplicor Mycobacterium tuberculosis kit.

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Point-Of-Care Testing for Tuberculosis

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