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      Pristimerin Suppresses Trophoblast Cell Epithelial–Mesenchymal Transition via miR-542-5p/EGFR Axis

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          Abstract

          Background

          Ectopic pregnancy (EP) is an ectopic embryo implantation occurred outside the uterine cavity. Nowadays, more attention have garnered in fast and effective treatment with less side effects. Pristimerin is known as the clinical application for anti-cancer, and the effect on EP therapy is still unclear.

          Materials and Methods

          Trophoblast cell line HTR-8/SVneo was used; then, we performed cell counting kit-8 assay, wound healing assay, flow cytometry and real-time polymerase chain reaction analysis (RT-PCR) to detect the cell viability, migration ability, apoptosis and epithelial–mesenchymal transition (EMT) under pristimerin treatment. In addition, public bioinformatic database was used to discover the connection between molecular and genes. Finally, we used miRNA transfection and RT-PCR techniques to determine the underlying molecular mechanism.

          Results

          We revealed that pristimerin inhibited trophoblast cells proliferation, migration and EMT, while induced trophoblast cell apoptosis. Furthermore, expression of miR-542-5p, AGO2 and EGFR was suppressed in HTR-8/SVneo cells post pristimerin treatment, and miR-542-5p silence showed the same effect. Combing pristimerin treatment and miR-542-5p silence showed a synergistic action.

          Conclusion

          Pristimerin could be an effective treatment to block embryo implantation by miR-542-5p and EGFR down-regulation.

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          Most cited references 59

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            Global classification of the human proteins with regards to spatial expression patterns across organs and tissues is important for studies of human biology and disease. Here, we used a quantitative transcriptomics analysis (RNA-Seq) to classify the tissue-specific expression of genes across a representative set of all major human organs and tissues and combined this analysis with antibody-based profiling of the same tissues. To present the data, we launch a new version of the Human Protein Atlas that integrates RNA and protein expression data corresponding to ∼80% of the human protein-coding genes with access to the primary data for both the RNA and the protein analysis on an individual gene level. We present a classification of all human protein-coding genes with regards to tissue-specificity and spatial expression pattern. The integrative human expression map can be used as a starting point to explore the molecular constituents of the human body.
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              The emergence of microRNAs has been one of the defining developments in cancer biology over the past decade, and the explosion of knowledge in this area has brought forward new diagnostic and therapeutic opportunities. The importance of microRNAs in cancer has been underlined by the identification of alterations in microRNA target binding sites and the microRNA processing machinery in tumor cells. Clinical trials utilizing microRNA profiling for patient prognosis and clinical response are now underway, and the first microRNA mimic entered the clinic for cancer therapy in 2013. In this article we review the potential applications of microRNAs for the clinical assessment of patient outcome in cancer, as well as in cancer monitoring and therapy. Copyright © 2014 Elsevier Ltd. All rights reserved.
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                Author and article information

                Journal
                Drug Des Devel Ther
                Drug Des Devel Ther
                dddt
                dddt
                Drug Design, Development and Therapy
                Dove
                1177-8881
                02 November 2020
                2020
                : 14
                : 4659-4670
                Affiliations
                [1 ]Department of Obstetrics and Gynecology, The First Hospital of Jilin University , Changchun, Jilin 130021, People’s Republic of China
                [2 ]Centre for Reproductive Medicine, Centre for Prenatal Diagnosis, The First Hospital of Jilin University , Changchun, Jilin 130021, People’s Republic of China
                [3 ]Department of Rehabilitation, The First Hospital of Jilin University , Changchun, Jilin 130021, People’s Republic of China
                [4 ]Central Laboratory, The First Hospital of Jilin University , Changchun, Jilin 130021, People’s Republic of China
                [5 ]Department of Cardiology, The First Hospital of Jilin University , Changchun, Jilin 130021, People’s Republic of China
                Author notes
                Correspondence: Cong Hu; Bo Pang Email conghu@jlu.edu.cn bopang@jlu.edu.cn
                [*]

                These authors contributed equally to this work

                Article
                274595
                10.2147/DDDT.S274595
                7646443
                © 2020 Shu et al.

                This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License ( http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms ( https://www.dovepress.com/terms.php).

                Page count
                Figures: 5, Tables: 2, References: 59, Pages: 12
                Funding
                Funded by: Finance Department of Jilin Province;
                Funded by: Education Department of Jilin Province;
                The research is supported by grant from NSFC (81671592), Finance Department of Jilin Province (JLSWSRCZX2020-089), Education Department of Jilin Province (JJKH20201064KJ), and Science and Technology Department of Jilin Province (20180201028YY).
                Categories
                Original Research

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