Blog
About

  • Record: found
  • Abstract: found
  • Article: not found

Isolation of intracellular membranes by means of sodium carbonate treatment: application to endoplasmic reticulum

The Journal of Cell Biology

The Rockefeller University Press

Read this article at

ScienceOpenPMC
Bookmark
      There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

      Abstract

      A rapid and simple method for the isolation of membranes from subcellular organelles is described. The procedure consists of diluting the organelles in ice-cold 100 mM Na2CO3 followed by centrifugation to pellet the membranes. Closed vesicles are converted to open membrane sheets, and content proteins and peripheral membrane proteins are released in soluble form. Here we document the method by applying it to various subfractions of a rat liver microsomal fraction, prepared by continuous density gradient centrifugation according to Beaufay et al. (1974, J. Cell Biol. 61:213-231). The results confirm and extend those of previous investigators on the distribution of enzymes and proteins among the membranes of the smooth and rough endoplasmic reticulum. In the accompanying paper (1982, J. Cell Biol. 93:103-110) the procedure is applied to peroxisomes and mitochondria.

      Related collections

      Author and article information

      Journal
      J Cell Biol
      The Journal of Cell Biology
      The Rockefeller University Press
      0021-9525
      1540-8140
      1 April 1982
      : 93
      : 1
      : 97-102
      2112113
      82167809
      7068762
      Categories
      Articles

      Cell biology

      Comments

      Comment on this article