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      Comparative evaluation of antibody positive titer by ELISA and IFA inTheileria annulatavaccinated cattle in Iran

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      Parasite

      EDP Sciences

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          Abstract

          An enzyme linked immunosorbent assay (ELISA) was used to evaluate antibody positive titer in vaccinated and non-vaccinated cattle using schizont infected myeloid cells as an antigen. The result was compared with indirect fluorescent antibody level in the same animals. For this study 116 milking cows, 95 vaccinated and 21 non-vaccinated, were bleeded in order to prepare sera. They were tested with both ELISA and IFA tests. 94 sera had positive antibody titer and 22 sera were negative through ELISA test but, with IFA test, only 89 sera showed positive antibody titer and 27 were negative. Thereby, it was concluded that the sensitivity and specificity of ELISA test in comparison with IFA test was 95.5% and 66.6% respectively. This study generally indicated that ELISA could be an effective test for sero-epidemiological investigations of bovine tropical theileriosis, and it is considered to be valid as an additional test to distinguish the vaccinated from the non vaccinated cattle in order to schedule vaccination programs.

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          Author and article information

          Journal
          Parasite
          Parasite
          EDP Sciences
          1252-607X
          1776-1042
          March 2006
          March 2006
          : 13
          : 1
          : 71-74
          Article
          10.1051/parasite/2006131071
          16605070
          © 2006

          This work is licensed under a Creative Commons Attribution 4.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/

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          Self URI (journal page): http://www.parasite-journal.org/

          Parasitology, Life sciences

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