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      Human malaria parasites in continuous culture.

      Science (New York, N.Y.)

      Animals, Cells, Cultured, Culture Media, Erythrocytes, parasitology, Humans, In Vitro Techniques, Plasmodium falciparum, growth & development, Reproduction, Asexual

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          Abstract

          Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38 degrees C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen (1 or 5 percent). The original parasite material, derived from an infected Aotus trivirgatus monkey, was diluted more than 100 million times by the addition of human erythrocytes at 3- or 4-day intervals. The parasites continued to reproduce in their normal asexual cycle of approximately 48 hours but were no longer highly synchronous. The have remained infective to Aotus.

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          781840

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