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      In vitro reproductive toxicity of polychlorinated biphenyl congeners 153 and 126.

      Reproductive Toxicology (Elmsford, N.y.)
      Animals, Blastocyst, drug effects, Cattle, Dose-Response Relationship, Drug, Embryo, Mammalian, Female, In Vitro Techniques, Oocytes, Polychlorinated Biphenyls, toxicity, Reproductive Medicine, methods

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          Abstract

          This study was conducted to investigate the applicability of an in vitro technique for maturation, fertilization, cleavage, and growth to blastocysts of bovine oocytes to investigate reproductive toxicologic effects. During maturation, the oocytes were exposed to the di-ortho-substituted PCB congener 2,2',4,4',5,5'-CB (PCB 153) in the three concentrations 0.84 ng/mL, 8.4 ng/mL, and 84 ng/mL or to the non-ortho-substituted PCB congener 3,3'4,4',5-CB (PCB 126) in the three concentrations 1.006 pg/mL, 10.06 pg/mL, and 100.6 pg/mL and compared with control groups. PCB 153 had no effect on maturation but resulted in a reduced proportion of oocytes that cleaved at the highest concentration. There were no differences in blastocyst development among groups. PCB 126 resulted in a reduction in maturation percentage at the highest concentration and in blastocyst development at all concentrations. These results demonstrated adverse effects of PCB congeners on bovine oocytes and showed that this system can be used to evaluate toxic effects on oocytes and preimplantation-stage embryos.

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