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      Adenine base editors catalyze cytosine conversions in human cells

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          Abstract

          <p xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" class="first" dir="auto" id="d972996e172">Adenine base editors comprise an adenosine deaminase, evolved in vitro, and a Cas9 nickase. Here, we show that in addition to converting adenine to guanine, adenine base editors also convert cytosine to guanine or thymine in a narrow editing window (positions 5-7) and in a confined TC*N sequence context. Adenine base editor-induced cytosine substitutions occur independently of adenosine conversions with an efficiency of up to 11.2% and reduce the number of suitable targeting sites for high-specificity base editing. </p>

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          Author and article information

          Journal
          Nature Biotechnology
          Nat Biotechnol
          Springer Science and Business Media LLC
          1087-0156
          1546-1696
          September 23 2019
          Article
          10.1038/s41587-019-0254-4
          31548727
          05f1e982-e7b8-4885-90f7-059cfa7194a1
          © 2019

          http://www.springer.com/tdm

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