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      AB183. Therapeutic effects of adipose-derived stem cells-based micro-tissues on erectile dysfunction in streptozotocin-induced diabetic rats

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          Abstract

          Objective

          This study aimed to explore the therapeutic effects of adipose-derived stem cells (ADSCs)-based micro-tissues (MTs) on erectile dysfunction (ED) in streptozotocin (STZ) induced diabetic rats.

          Methods

          Fifty-eight 8-week-old Sprague-Dawley rats received intraperitoneal injection of STZ (60 mg/kg) and eight weeks later, the determined diabetic rats randomly got intracavernous (IC) injection of phosphate buffer solution (PBS), ADSCs and MTs. Another eight normal rats equally received IC injection of PBS. The injected cells were tracked in ADSCs and MTs groups. Four weeks after the treatment, intracavernous pressure (ICP), histopathological changes in corpus cavernosum (CC), apoptotic index and functional proteins were measured.

          Results

          MTs expressed vascular endothelial growth factor (VEGF), nerve growth factor (NGF), tissue inhibitor of metallopeptidase-1 (TIMP-1) and tumour necrosis factor-stimulated gene 6 (TSG-6). MTs had a better retention than ADSCs and MTs treatment improved ICP, neuronal nitric oxide synthase (nNOS) expression, smooth muscle and endothelial content in diabetic rats, ameliorated apoptosis and local inflammation in CC.

          Conclusions

          The results suggested that IC injection of MTs improves the erectile function and histopathological changes in STZ-induced diabetic rats and appears to be more promising than traditional ADSCs. The underlying mechanisms involve increased cell retention accompanied with neuroprotection and anti-inflammatory behaviors of the paracrine factors.

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          Author and article information

          Journal
          Transl Androl Urol
          Transl Androl Urol
          TAU
          Translational Andrology and Urology
          AME Publishing Company
          2223-4691
          August 2015
          August 2015
          : 4
          : Suppl 1
          : AB183
          Affiliations
          The First Affiliated Hospital of Soochow University, Soochow 215006, China; Center of Andrology, Peking University First Hospital, Beijing 100034, China; General Hospital of Ningxia Medical University, Yinchuan 750004, China
          Article
          tau-04-S1-AB183
          10.3978/j.issn.2223-4683.2015.s183
          4708785
          0618c6f6-fea1-4755-9bf6-a6112f1429db
          2015 Translational Andrology and Urology. All rights reserved.
          History
          Categories
          Moderated Poster Presentation

          diabetes mellitus,erectile dysfunction (ed),adipose-derived stem cells (adscs),micro-tissues (mts)

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