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      DNA polymorphism in Mycobacterium paratuberculosis, "wood pigeon mycobacteria," and related mycobacteria analyzed by field inversion gel electrophoresis.

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          Abstract

          Mycobacterium paratuberculosis strains, mycobacteria from patients suffering from Crohn's disease, "wood pigeon mycobacteria," and representatives of Mycobacterium avium-Mycobacterium intracellulare were compared by restriction endonuclease DraI digestion and field inversion gel electrophoresis. Characteristic profiles were seen for M. paratuberculosis, including isolates from patients suffering from Crohn's disease, for wood pigeon mycobacteria, and for M. avium-M. intracellulare serotypes 2, 16, 18, and 19. Two M. paratuberculosis strains used for vaccine production (St 18 and 316 F) presented patterns different from those of the other M. paratuberculosis strains. Strains St 18 yielded a pattern identical to that of the M. avium type strain serotype 2, whereas 316 F gave a unique pattern. The method developed in this study represents a useful taxonomic tool for the identification and classification of mycobacteria.

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          Author and article information

          Journal
          J. Clin. Microbiol.
          Journal of clinical microbiology
          0095-1137
          0095-1137
          Dec 1989
          : 27
          : 12
          Affiliations
          [1 ] Unité de la Tuberculose et des Mycobactéries, Institut Pasteur, Paris, France.
          Article
          267134
          2574186
          0620a067-3b76-4425-8cb9-eace74985efd
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