For Publishers
DiscoveryMetadataPeer reviewHostingPublishing
For Researchers
JoinPublishReviewCollect
Register
Blog
About
Search
Advanced search
My ScienceOpen
Search
For Publishers
For Researchers
Blog
About
22
views
21
references
 Top references
cited by
9
    
0 reviews
 Review
0
comments
 Comment
0
recommends
+1 Recommend
0 collections
    0
    shares
      198
      similar
       All similar
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      HPLC-PDA-MS/MS Characterization of Bioactive Secondary Metabolites from Turraea fischeri Bark Extract and Its Antioxidant and Hepatoprotective Activities In Vivo

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Turraea fischeri is an East African traditional herb, which is widely used in traditional medicine. In this study, we profiled the secondary metabolites in the methanol extract of T. fischeri bark using HPLC-PDA-ESI-MS/MS, and 20 compounds were tentatively identified. Several isomers of the flavonolignan cinchonain-I and bis-dihydroxyphenylpropanoid-substituted catechin hexosides dominated the extract. Robust in vitro and in vivo antioxidant properties were observed in 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay (DPPH) and ferric reducing antioxidant power (FRAP) assay, and in the model organism Caenorhabditis elegans. Additionally, the extract exhibited promising hepatoprotective activities in D-galactosamine (D-GaIN) treated rats. A significant reduction in the elevated levels of aspartate aminotransferase (AST), total bilirubin, gamma-glutamyltransferase (GGT), and malondialdehyde (MDA) and increase of glutathione (GSH) was observed in rats treated with the bark extract in addition to D-galactosamine when compared with rats treated with D-galactosamine alone. In conclusion, T. fischeri is apromising candidate for health-promoting and for pharmaceutical applications.

          Related collections

          Most cited references21

          • Record: found
          • Abstract: found
          • Article: not found

          Epigallocatechin gallate from green tea (Camellia sinensis) increases lifespan and stress resistance in Caenorhabditis elegans.

          S Abbas, Michael Wink (2009)
          Epigallocatechin gallate (EGCG) is a major green tea polyphenol with pronounced antioxidative activity. The effects of EGCG on lifespan and stress resistance in wild-type N2 and transgenic strains of Caenorhabditis elegans [ HSP-16.2/GFP, MEV-1(KN1), FEM-1(HC17)] were investigated. The expression of HSP-16.2 (induced by the pro-oxidant juglone) and the intracellular levels of H (2)O (2) were inhibited by EGCG treatment. Daily administration of 220 muM EGCG increased the mean lifespan by 10.14 % and 14.27 % in N2 and FEM-1(HC17) strains, respectively, and 55 muM EGCG increased the mean lifespan in MEV-1(KN1) by 16.11 %. The survival rate was also increased under lethal oxidative stress by 65.05 %. These findings suggest that the increased mean lifespan and stress resistance in C. ELEGANS apparently depend, among other factors, on the antioxidant properties of EGCG.
          Article 0 comments Cited 65 times – based on 0 reviews     Review now
            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            Structural features and bioavailability of four flavonoids and their implications for lifespan-extending and antioxidant actions in C. elegans.

            Gregor Grünz, Kerstin Haas, Sebastian T. Soukup … (2012)
            Various studies have demonstrated longevity effects of flavonoids, a major sub-group of plant polyphenolic compounds, in Caenorhabditis elegans. To better understand their structure-activity relationship in vivo we have used a comparative approach by exposing C. elegans to the structurally related flavonoids myricetin, quercetin, kaempferol and naringenin, and assessed their impact on lifespan and on putative modes of action. The bioavailability of the tested flavonoids was demonstrated by high-performance liquid chromatography with diode-array detection (HPLC/DAD) and a 2-aminoethyl diphenyl borate-based in vivo approach. While all flavonols increased lifespan in wild-type, only myricetin elongated the mev-1(kn1) lifespan, suggesting that the flavonols antioxidant action alone is not sufficient for longevity. Structural prerequisites of high antioxidant action in vitro were also essential to reduce the reactive oxygen species (ROS) load in vivo in C. elegans and were tested in isolated mouse muscle mitochondria. Since the insulin/IGF-like signaling (IIS) cascade is a key regulator of lifespan, all compounds were tested for the ability to cause nuclear translocation of the FOXO transcription factor DAF-16 and changes in target gene expression. An increased DAF-16 translocation and sod-3 promoter activity were observed with all flavonoids but was independent of their ROS scavenging capability and their effects on lifespan. Copyright © 2011 Elsevier Ireland Ltd. All rights reserved.
            Article 0 comments Cited 50 times – based on 0 reviews     Review now
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Epigallocatechin gallate inhibits beta amyloid oligomerization in Caenorhabditis elegans and affects the daf-2/insulin-like signaling pathway.

              S Abbas, M. Wink (2010)
              Epidemiological studies have repeatedly demonstrated that green tea protects against oxidative stress involved in many diseases. Health benefits of green tea are attributed to its principal active constituent, epigallocatechin gallate (EGCG). EGCG was shown to increase the stress resistance and lifespan of Caenorhabditis elegans. The mechanism of this action has been investigated in this study. The expression of hsp-16.1 and hsp-16.2 in EGCG-treated worms (N2), as quantified by real-time PCR, was significantly lower under oxidative stress induced by juglone than in controls without EGCG. In the strain TJ356 (DAF-16::GFP) EGCG treatment induced translocation of DAF-16 from the cytoplasm into the nucleus, suggesting that EGCG may affect the daf-2/insulin-like signaling pathway. EGCG decreased the formation of lipofuscin, an aging related pigment. Also, EGCG reduced beta amyloid (Abeta) deposits and inhibited Abeta oligomerization in transgenic C. elegans (CL2006). Thus, the use of green tea and EGCG is apparently rational alternatives for protecting against ROS-mediated and age-related diseases. Copyright 2010 Elsevier GmbH. All rights reserved.
              Article 0 comments Cited 46 times – based on 0 reviews     Review now
                Bookmark
                All references

                Author and article information

                Journal
                Journal ID (nlm-ta): Molecules
                Journal ID (iso-abbrev): Molecules
                Journal ID (publisher-id): molecules
                Title: Molecules : A Journal of Synthetic Chemistry and Natural Product Chemistry
                Publisher: MDPI
                ISSN (Electronic): 1420-3049
                Publication date (Electronic): 29 November 2017
                Publication date Collection: December 2017
                Volume: 22
                Issue: 12
                Electronic Location Identifier: 2089
                Affiliations
                [1 ]Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg 69120, Germany; Dmirieh@ 123456stud.uni-heidelberg.de
                [2 ]Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt; mona_pharmacology@ 123456yahoo.com
                [3 ]Department of Pharmacognosy, College of Pharmacy, Cairo University, Cairo 11562, Egypt; omar.sabry@ 123456pharma.cu.edu.eg
                [4 ]Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt; rashaadelelfeqy@ 123456gmail.com (R.A.); assemels2002@ 123456yahoo.co.uk (A.M.E.-S.)
                Author notes
                [* ]Correspondence: sobeh@ 123456uni-heidelberg.de (M.S.); wink@ 123456uni-heidelberg.de (M.W.); Tel: +49-6221-54-4880 (M.W.); Fax: +49-6221-54-4884 (M.W.)
                Author information
                https://orcid.org/0000-0002-7875-4510
                Article
                Publisher ID: molecules-22-02089
                DOI: 10.3390/molecules22122089
                PMC ID: 6149860
                PubMed ID: 29186050
                SO-VID: 06400c88-6922-4474-a57e-58431c12548b
                Copyright © © 2017 by the authors.
                License:

                Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license ( http://creativecommons.org/licenses/by/4.0/).

                History
                Date received : 06 November 2017
                Date accepted : 27 November 2017
                Categories
                Subject: Article

                Keywords: turraea fischeri,flavonolignan,cinchonains,hplc-pda-esi-ms/ms,antioxidant,hepatoprotection
                Data availability:
                Keywords: turraea fischeri, flavonolignan, cinchonains, hplc-pda-esi-ms/ms, antioxidant, hepatoprotection

                Comments

                Comment on this article

                scite_

                Similar content198

                See all similar

                Cited by9

                See all cited by

                Most referenced authors313

                See all reference authors