23
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Hevin is down-regulated in many cancers and is a negative regulator of cell growth and proliferation

      research-article

      Read this article at

      ScienceOpenPublisherPMC
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          We have cloned a human Hevin cDNA from omental adipose tissue of different patients by reverse transcription polymerase chain reaction and shown a sequence variation due to a possible polymorphism at amino acid position 161 (E/G). Hevin protein expressed in vitro showed molecular weights of approximately 75 kDa and 150 kDa, suggesting that Hevin may form a homodimer in vitro. Using Northern blots and a human expressed sequence tAg database analysis, Hevin was shown to be widely expressed in human normal or non-neoplastic diseased tissues with various levels. In contrast to this, its expression was strongly down-regulated in most neoplastic cells or tissues tested. However, neither the mechanism nor the physiological meaning of this down-regulation is known. As an initial step towards investigating the functional role of Hevin in cell growth and differentiation, we transiently or stably expressed this gene in cancer cells (HeLa 3S) that are devoid of endogenous Hevin and measured DNA synthesis (cell proliferation) by 5-bromo-2′-deoxyuridine incorporation. Hevin was shown to be a negative regulator of cell proliferation. Furthermore, we have shown that Hevin can inhibit progression of cells from G1 to S phase or prolong G1 phase. This is the first report which describes the function of Hevin in cell growth and proliferation. Through database analysis, Hevin was found to be located on chromosome 4 which contains loss of heterozygosity of many tumour suppressor genes. Taken together, these results suggest that Hevin may be a candidate for a tumour suppressor gene and a potential target for cancer diagnosis/therapy. © 2000 Cancer Research Campaign

          Related collections

          Author and article information

          Journal
          Br J Cancer
          British Journal of Cancer
          Nature Publishing Group
          0007-0920
          1532-1827
          21 February 2000
          March 2000
          : 82
          : 6
          : 1123-1130
          Affiliations
          [1 ]Department of Biochemistry, [2 ]Biotechnology, [3 ]Immunology, Janssen Research Foundation, Turnhoutseweg 30, Beerse, B-2340, Belgium
          [4 ]Department of Surgery, St Jozef Hospital, Stw op Merksplas 44, Turnhout, B-2200, Belgium
          Article
          6691051
          10.1054/bjoc.1999.1051
          2363342
          10735494
          066839ca-31fd-43b1-a6a9-aa37295d762d
          Copyright 2000, Cancer Research Campaign
          History
          : 09 February 1999
          : 13 October 1999
          : 13 October 1999
          Categories
          Regular Article

          Oncology & Radiotherapy
          cancer,cell cycle,tumor suppressor gene,hevin,cell proliferation
          Oncology & Radiotherapy
          cancer, cell cycle, tumor suppressor gene, hevin, cell proliferation

          Comments

          Comment on this article