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      Rapid and Simple Determination of the Escherichia coli Phylogenetic Group

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      Applied and Environmental Microbiology
      American Society for Microbiology

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          Abstract

          Phylogenetic analysis has shown that Escherichia coli is composed of four main phylogenetic groups (A, B1, B2, and D) and that virulent extra-intestinal strains mainly belong to groups B2 and D. Actually, phylogenetic groups can be determined by multilocus enzyme electrophoresis or ribotyping, both of which are complex, time-consuming techniques. We describe a simple and rapid phylogenetic grouping technique based on triplex PCR. The method, which uses a combination of two genes (chuA and yjaA) and an anonymous DNA fragment, was tested with 230 strains and showed excellent correlation with reference methods.

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          Author and article information

          Journal
          Applied and Environmental Microbiology
          Applied and Environmental Microbiology
          American Society for Microbiology
          0099-2240
          October 01 2000
          October 01 2000
          : 66
          : 10
          : 4555-4558
          Article
          10.1128/AEM.66.10.4555-4558.2000
          92342
          11010916
          068a0b1c-7242-4bd7-bca5-b2954321b75b
          © 2000
          History

          Molecular medicine,Neurosciences
          Molecular medicine, Neurosciences

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