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      Expression profiling reveals off-target gene regulation by RNAi.

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          Abstract

          RNA interference is thought to require near-identity between the small interfering RNA (siRNA) and its cognate mRNA. Here, we used gene expression profiling to characterize the specificity of gene silencing by siRNAs in cultured human cells. Transcript profiles revealed siRNA-specific rather than target-specific signatures, including direct silencing of nontargeted genes containing as few as eleven contiguous nucleotides of identity to the siRNA. These results demonstrate that siRNAs may cross-react with targets of limited sequence similarity.

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          Author and article information

          Journal
          Nat Biotechnol
          Nature biotechnology
          Springer Science and Business Media LLC
          1087-0156
          1087-0156
          Jun 2003
          : 21
          : 6
          Affiliations
          [1 ] Rosetta Inpharmatics LLC, 12040 115th Avenue NE, Kirkland, Washington 98034, USA. aimee_jackson@merck.com
          Article
          nbt831
          10.1038/nbt831
          12754523
          06d515cd-ef4e-4830-a507-b518ba13b149
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