The biospeleological investigations of several cenotes in the eastern region of Yucatán state, Mexico, during January 2018 yielded, among other invertebrates, two new truncatelloid gastropod species described herein as Mexicenoticaxochii gen. n. et sp. n. and Pyrgophorusthompsoni sp. n. Both species represent the first record of stygobiont gastropod species from the cenotes of Yucatán indicating the high biodiversity potential of the studied area.