The percentage of murine hematopoietic stem and progenitor cells, which present with
a loss of function upon treatment with the genotoxic agent hydroxyurea, is inversely
correlated to the mean lifespan of inbred mice, including the long-lived C57BL/6 and
short-lived DBA/2 strains. Quantitative trait locus mapping in BXD recombinant inbred
strains identified a region spanning 12.5 cM on the proximal part of chromosome 11
linked to both the percentage of dysfunctional hematopoietic stem and progenitor cells
as well as regulation of lifespan. By generating and analyzing reciprocal congenic
mice for this locus, we demonstrate that this region indeed determines the sensitivity
of hematopoietic stem and progenitor cells to hydroxyurea. These cells do not present,
as previously anticipated, with differences in cell cycle distribution; neither do
they present with changes in the frequency of cells undergoing apoptosis, senescence,
replication stalling and re-initiation activity, excluding the possibility that variations
in proliferation, replication or viability underlie the distinct response of these
cells from the congenic and parental strains. An epigenetic aging clock in blood cells
was accelerated in C57BL/6 mice congenic for the DBA/2 version of the locus. We verified
pituitary tumor-transforming gene-1 (Pttg1)/Securin as the quantitative trait gene
regulating the differential response of hematopoietic stem and progenitor cells to
hydroxyurea treatment and which might therefore be linked to the regulation of lifespan.
Introduction
We previously reported a correlation between the frequency of hematopoietic stem and
progenitor cells (HSPC) from a set of inbred mouse strains with impaired progenitor
cell function upon treatment with hydroxyurea (HU) and the mean lifespan of these
mice. The set of inbred strains also included C57BL/6 (B6) (low frequency of HSPC
dysfunctional in response to HU, long lifespan) and DBA/2 (D2) (high frequency of
HSPC dysfunctional in response to HU, short lifespan). In these experiments, the in
vitro cobblestone area forming cell (CAFC) assay was used to determine the number
of functional HSPC before and after treatment with HU. Given that HU kills proliferating
cells via the induction of DNA strand breaks that arise from stalled replication forks
after depletion of the nucleotide pool, this finding was interpreted as a significantly
higher percentage of HSPC from D2 versus B6 in S-phase, and subsequently that elevated
levels of HSPC proliferation could be negatively linked to lifespan.
1–3
Using BXD recombinant inbred (RI) mice, which are genetic chimeras based on B6 and
D2, subsequently a quantitative trait locus (QTL) was mapped to chromosome 11 linked
to the frequency of HSPC susceptible to HU. Interestingly, the same locus showed also
a linkage to the mean lifespan within the BXD RI set of mice, transforming the reported
phenotypic correlation into a genetic connection, implying a common underlying gene
and thus mechanism for the regulation of both phenotypes. To verify the linkage, and
identify the underlying quantitative trait gene, we generated B6 as well as D2 mice
that are reciprocally con-genic for this locus on chromosome 11.
Methods
Mice
Laboratory C57BL/6J (B6), DBA/2J (D2) and BXD inbred mice were obtained from Janvier
Labs (France). All mice were fed acidified water and food ad libitum, and housed under
pathogen-free conditions at the University of Kentucky, Division of Laboratory Animal
Resource, the animal facility at CCHMC. Mouse experiments were performed in compliance
with the German Law for Welfare of Laboratory Animals and were approved by the Regierungspräsidium
Tübingen or approved by the IACUCs of the University of Kentucky and CCHMC.
Quantitative trait locus mapping
Linkage analysis and determination of the likelihood ratio statistic values for suggestive
linkage were performed as described by using WebQTL (
http://www.genenetwork.org/webqtl/main.py?FormID=submitSingleTrait
), identifying the restrictive chromosome 11 locus, among others, correlating to mean
life span and HU sensitivity.
3–6
Generation of congenic mice
Congenic animals were generated in five generations by a marker-assisted backcrossing
strategy as described
3,5,7–9
(Figure 1C). The particular DBA/2J genomic region was derived from BXD31, one of the
BXD recombinant inbred strains used in the quantitative trait locus (QTL) mapping
and which phenotypically best demonstrated the decline in HSC in old age and the HU
sensitivity.
3
Figure 1.
Quantitative trait locus (QTL) analysis of hydroxyurea (HU) responses and mean life
spans of BXD mice and generation of mice congenic for the corresponding chromosome
11 locus. (A) WebQTL analysis of HU sensitivity rates and mean life spans of hematopoietic
stem and progenitor cells (HSPC) isolated from various BXD and parental strains, identifying
a proximal part of chromosome 11, among others, involved in this phenotype. Values
are in Likelihood Ratio Statistics (LRS). (B) QTL analysis of mean life spans and
HU responses of the various BXD strains for chromosome 11. (C) Schematic illustration
showing the generation of the congenic mouse strains line A and K. Briefly, after
crossing B6 with D2 mice, F1 littermates were backcrossed with the corresponding parental
strains (B6/D2). Offspring were backcrossed in four rounds with parental strains reciprocal
for the corresponding chromosome 11 specific SNP D11Mit20 to finally obtain B6 or
D2 mice congenic for the proximal locus on chromosome 11 of D2 or B6, respectively.
(D) SNP analysis of chromosome 11 from strains B6, D2, A and K.
Preparation of hematopoietic tissue and cells
For the isolation of total bone marrow (BM), tibiae, femur and hips of mice were isolated
and flushed using a syringe and a G21 needle. Mononuclear (low density bone marrow,
LDBM) cells were isolated by Histopaque low-density centrifugation (#10831, Sigma).
Lineage depletion was performed using the mouse lineage cell depletion Kit (#130-090-858,
Miltenyi Biotec) according to their protocol.
Cobblestone area forming cell assay
Cobblestone area forming cell (CAFC) assay was performed as described.
1
Briefly, 1,000 FBMD-1 cells, a stromal cell line, were seeded in each well of 96-well
plates. Plates were incubated at 33°C in 5% C02, and used seven days later for CAFC
assay. BM cells were either treated with 200 mg/mL HU or its solvent (PBS) and seeded
onto the pre-established stromal layers in six dilutions, serially in 3-fold increments
from 333 to up to 81,000 cells/well (12 wells per dilution). At this time, the medium
was switched from 5% horse serum and 10% fetal bovine serum to 20% horse serum. Alternatively,
mice were treated with HU in vivo as indicated following bone marrow isolation and
seeding. After seven days, all wells were evaluated for the presence or absence of
cobblestone areas and the frequency of the appearance of a colony calculated using
L-Calc software (STEMCELL Technologies).
Analysis of the epigenetic aging signature
Analysis of DNA methylation levels was analyzed at three age-associated CG dinucleotides
(CpG) as described previously.
10
Briefly, genomic DNA was isolated from blood samples, bisulfite converted, and DNA
methylation was analyzed within the three genes (Prima1, Hsf4, Kcns1) by pyrosequencing.
The DNA methylation results at these sites can be integrated into a multivariable
model for epigenetic age predictions in B6 mice, which clearly correlate with the
chronological age.
10
Statistical analysis
All statistical analyses were performed using Student’s t-test or two-way Anova, when
appropriate with GraphPad Prism 6 software. For Figure 4C, linear and non-linear regression
was calculated. The number of biological repeats (n) is indicated in the figure legends.
Error bars are Standard Error of Mean (SEM).
Results
Hematopoietic stem and progenitor cells from BXD RI strains show highly divergent
reactions when exposed to HU as judged by their ability to form cobblestones on stromal
feeder layers in the CAFC assay after seven days of culture (CAFC day 7 assay).
9
Re-analyzing the initial phenotypic data based on the most recent marker map (New
Genotypes 2017 dataset) provided for BXD RI strains, we verified the initially identified
locus on chromosome 11 (35-75 Mb) linked (with a suggestive threshold of 10.53/10.88)
to both HU susceptibility of HSPC as well as mean lifespan of the analyzed mice (Figure
1A and B and Online Supplementary Tables S1A and B, and S3). We used a marker assisted
speed congenic approach to obtain a reciprocal set of mice congenic for the chromosome
11 locus (Figure 1C). These novel mouse lines were named line A (D2 onto B6) and K
(B6 onto D2). We performed whole genome SNP mapping of our congenic mouse strains
to identify the length of the congenic intervals transferred as well as the overlap
between the reciprocal strains. Ultimately, the common region transferred in line
A and line K spans an 18.6 Mb (8.3 cM) region on chromosome 11 from rs26900200, 37,929,686
bp to rs3088940, 56,516,067 bp with no other transferred intervals stemming from the
donor strains that are identical between the two congenic strains. The SNP analysis
further revealed a small set of additional congenic regions in both line A and K animals,
though not covering identical regions (Figure 1D, Online Supplementary Table S2 and
Online Supplementary Figure S1). This interval contains about 130 protein coding genes
(Online Supplementary Table S3).
Next, based on the CAFC assay, we tested whether the genotype of the locus conferred
in the congenic strains correlated with the magnitude of our phenotype of HSPC susceptible
to HU. HU treatment efficiently suppresses BrdU incorporation and thus active S-Phase
in freshly isolated Lin-cKit+ (LK) cells from all strains (Online Supplementary Figure
S2A). Indeed, HSPC isolated from B6 or line K (B6 onto D2) mice presented with a lower
frequency of dysfunctional HSPC in response to short-term in vivo as well as to ex
vivo treatment with HU, while inversely, D2 and line A (D2 onto B6) HSPC were more
sensitive to HU (Figure 2A and Online Supplementary Figure S2B). These data confirm
that the interval on chromosome 11 shared among the congenic strains confers this
phenotype and might thus contain a gene regulating the response of HSPC to HU.
Figure 2.
The chromosome 11 locus controls sensitivity of hematopoietic stem and progenitor
cells (HSPC) to hydroxyurea (HU) exposure but not HSPC frequency, cell cycle activity,
apoptosis and replication fork stalling. (A) Mice from all four groups were injected
with 10 mg HU/kg body weight or its solvent (PBS) for 1 hour (h) following isolation
of bone marrow (BM) cells and processing for the cobblestone area-forming cell (CAFC)
assay. Shown is the fraction of HSPC sensitive to HU. n=5-12. (B) Cell cycle distributions
of HSPC (left), Lin-Sca1+cKit+ cells (LSK) (middle) and Lin−cKit+ cells (LK) (right)
of bromodeoxyuridine (BrdU)-treated mice. n=4. (C) Relative low density bone marrow
cells (LDBM) frequencies per tibia and femur of Lin−cKit+ cells (LK), Lin-Sca1+cKit+
cells (LSK) and hematopoietic stem cells (HSC) of the four mouse strains. n=4. (D)
(Left) LDBM cells from the four strains were treated with HU or its solvent (PBS)
for 1 h. Thereafter, LK cells (left) and HSC (right) were analyzed in terms of apoptosis
(AnnexinV). n=4. (E) LDBM cells were either treated with a control (−HU), HU for 1
h or accordingly following HU removal (RV) by washing twice with medium and an additional
resting period of 3 h (+HU RV). Thirty minutes prior to staining, all samples were
co-cultured with BrdU. (Left) Representative BrdU/7AAD FACS plots of LK cells from
the indicated strains. (Right) Quantification of LK cell cycle distribution. n=3.
(F) LK cells from all four mouse strains were either treated with a control, HU for
1 h or accordingly following HU RV and an additional resting period of 3 h (+HU RV).
Thereafter, cells were harvested and stained against gH2AX. (Left) Representative
confocal images. (Right) Quantification of the number of gH2AX foci per cell. n=3.
Significances are related to the corresponding −HU controls. *P<0.05; **P<0.01; ***P<0.001;
****P<0.0001.
Since HU inhibits dNTP synthesis,
11
and a lack of dNTP causes replication fork stalling and thus DNA damage and apoptosis,
12
it is believed that the frequency of cells susceptible to HU treatment is an indirect
measurement for the frequency of cells in the S-phase of the cell division cycle.
It has been thus concluded that the underlying mechanism of the distinct susceptibility
of HSPC from the inbred strains is due to distinct S-phase frequencies. BM cells with
the Lin-cKit+ surface marker combination (hematopoietic progenitor cells, LK cells)
are highly enriched for CAFC day 7 cells (Online Supplementary Figure S2C). However,
analysis of the frequency of LK cells from the inbred and the congenic strains in
different stages of the cell division cycle by in vivo BrdU incorporation and flow
cytometry, as well as that of hematopoietic stem cells (HSC) and less primitive progenitors
(LSK), revealed almost identical patterns and especially almost identical frequencies
of cells in S-phase among all the strains tested (Figure 2B). HU susceptibility in
HSPC does therefore not correlate with the frequency of HSPC in S-phase, which excludes
differences in cycling frequencies as the underlying mechanism for the phenotype observed,
as well as in general HU susceptibility as surrogate for the frequency of cells in
S-phase. Consistent with that finding was the fact that HSPC from all groups had similar
telomere lengths. Short telomeres can be seen as a surrogate marker for high levels
of proliferation (Online Supplementary Figure S2D). In addition, the frequency of
LK and LSK was very similar in all strains, while D2-derived mice displayed a general
higher HSC frequency, as already reported,
1
which is, however, not mirrored in B6/line A mice and thus locus-independent. That
finding excludes a difference in the number of these cells as a factor contributing
to the phenotype (Figure 2C). Furthermore, the frequencies of HSPC undergoing apoptosis
upon ex vivo HU treatment and under steady state conditions in vivo were at a low
level among these groups, even when regarding S-phase specific apoptosis rates as
well senescence in response to HU as indicated by the level of the senescence marker
p16 in HSPC (Figure 2D and Online Supplementary Figure S2E and F). In addition, whereas
HU treatment almost completely blocks BrdU incorporation, LK cells from all strains
preserve their ability to re-enter active S-phase in a locus-independent manner 3
and even 16 hours (h) after HU is removed, excluding the possibility that enhanced
levels of senescence, apoptosis or difference in re-initiation of replication after
stalling are causative for the HU sensitivity phenotype (Figure 2E and Online Supplementary
Figure S2G). Similarly, LK cells from all strains showed comparable frequencies of
gH2AX foci per cells upon HU treatment and 3 h post HU removal, which also excludes
a role of variation in stalling of replication and the subsequent DNA damage for our
phenotype (Figure 2F). In aggregation, these data exclude a likely contribution of
differences in cell cycle and replication parameters as well as differential senescence
or apoptosis to the highly unequal HU susceptibilities of HSPC in the inbred and congenic
strains, while the underlying mechanism still remains to be identified.
A D2-allele at the genetic microsatellite marker D11Mit174 (Chr.11:42,593,949-42,594,095,
which is within the area with the highest level of linkage) correlated in the BXD
RI set, as anticipated, with higher HU-susceptibility rates of HSPC and a lower mean
life span (Figure 3A). The gene Pttg1 (Securin), which has been reported to inhibit
mitotic division,
13,14
is located in very close proximity (+ 800 kB) to D11Mit174.
15
In addition, the yeast homolog of Securin, Pds1p, was reported to be critically involved
in the regulation of the intra-S-checkpoint and regulation of the response of yeast
to treatment with HU.
16
Previously, a 3-11-fold overexpression of Pttg1 in various D2 tissues compared to
B6 was demonstrated.
17–19
This renders Pttg1 a prime candidate quantitative trait gene in the interval on chromosome
11. To investigate whether the Pttg1 mediates the HU response, we analyzed its expression
in our experimental mouse strains. We observed a 3-5-fold increase in gene and protein
expression in D2 or line A derived HSPC compared to the corresponding cells from B6
or line K mice (Figure 3B and C). A D2-allele of the locus thus confers elevated expression
of Pttg1. Analyzing Pttg1-associated promoter and exon regions in silico revealed
a 7 bp insertion downstream of the transcription start (NCBI Reference Sequence: NC_000077.6)
in the D2 genome, potentially positively affecting binding of transcription factors
(TF) (Online Supplementary Figure S3A). Since the occurrence of these D2- and A/J-specific
7 bp was previously reported to result in reduced Pttg1 expression in contrast to
what we find in D2 animals,
20
we further determined the promoter structure of Pttg1 in more detail by polymerase
chain reaction (PCR) of genomic DNA. Surprisingly, the Pttg1 promoter region was present
in two differently sized versions (the two fragments differ in size by approx. 700
bp) in D2 and line A mice (Figure 3D). DNA sequencing revealed that the short version
in D2 (D2_1) was identical to the B6 Pttg1 promoter, while the longer version (D2_2)
was unique to D2 and included the already described 7 bp insertion in addition to
an additional 675 bp region between the transcription and the ORF start, which is
not completely annotated in common genome databases at the present time in contrast
to the 7 bp insertion (Online Supplementary Figure S3B and C). This could imply a
likely gene duplication of Pttg1 within the congenic locus. We next tested whether
the distinct types of promoter regions are causative for the dissimilar Pttg1 expression
patterns. By applying a dual-specific luciferase assay, we observed an almost 3-fold
increase in activity of the D2_2-specific promoter compared to the B6 and the shorter
D2_1 variants, suggesting that not the 7 bp insertion but the additional 675 bp region
drive elevated levels of Pttg1 expression in D2 or A cells (Figure 3E). We also identified
several exon-specific SNP causing amino acid substitutions in Pttg1. Using 3D in silico
models that predict the protein structure of PTTG1, no obvious difference in the structure
was observed between the B6 and D2 variants besides a slight increase in 310 helices,
a common secondary structure, which renders an additional contribution of the coding
SNP of Pttg1 to the phenotype less likely (Online Supplementary Figure S4A).
Figure 3.
Chromosome 11 associated Pttg1 has an altered promotor sequence in D2/A mice leading
to enhanced expression. (A) Mean life span (left) or hydrox-yurea (HU) sensitivity
rates of hematopoietic stem and progenitor cells (HSPC) (right) of BXD mouse strains
relative to the occurrence of the SNP D11Mit174. (B) Pttg1 gene expression in HSPC
from the indicated mouse strains. n=3. (C) PTTG1 protein expression in HSPC from the
four mouse strains. (Left) Representative western blot images. (Right) Quantification.
n=3. (D) Polymerase chain reaction analysis of genomic DNA from lines B6, D2, A and
K using the primers 5’NheI-B6/D2_PTTG1_pr1 and 3’EcoRV-B6/D2_PTTG1_pr2. Major bands
corresponding to the different promotors are indicated with arrows. (E) Dual-specific
luciferase assay for the indicated promotor constructs, including a negative (pNL1.1[Nuc])
and a positive (pNL1.1[CMV]) control. The corresponding constructs to Figure 3D are
highlighted in blue. n=3 (3 rounds with triplicates). *P<0.05; **P<0.01; ****P<0.0001.
To test whether Pttg1 is indeed the QTL gene within the described locus, and thus
whether the increased HU-sensitivity of HSPC is caused by elevated Pttg1 levels, we
overexpressed a Pttg1-Egfp fusion gene by lentiviral transduction in B6 HSPC. The
level of expression of the transgene was within the range of the difference in gene
expression between B6 and D2 HSPC and thus in a physiological range (Figure 4A, left
panel). Transduced BM cells were transplanted into B6 recipients for their in vivo
expansion. We sorted GFP+ BM cells five weeks post transplantation to analyze the
susceptibility of HSPC to HU with the CAFC assay. BM cells of the transplanted mice
were presented with similar rates of transduction (GFP+ cells), excluding a potential
bias of certain subpopulations upon transduction (Online Supplementary Figure S4B
and C). Elevated expression of Pttg1 in B6 HSPC resulted in a significant increase
in their susceptibility to HU treatment (Figure 4A, right panel). Similarly, upon
downregulation of Pttg1 in progenitor cells from line A and D2 mice, we observed a
trend towards reduced HU sensitivity (Online Supplementary Figure S4D). These data
confirm a causative role for distinct levels of expression of Pttg1 for the susceptibility
of HSPC to short-term HU treatment, and thus strongly imply that Pttg1 is the QTL
gene within the QTL locus.
Figure 4.
Pttg1 promotes hydroxyurea (HU) sensitivity of hematopoietic stem and progenitor cells
(HSPC) and influences epigenetic aging. (A) HSPC from B6 mice were cytokine-stimulated
and transduced with lentiviruses mediating stable endogenous Pttg1-Egfp (PTTG1 OE)
or Egfp (control) overexpression. After transplantation into B6 recipients, total
BM GFP+ cells were isolated, treated with HU or its solvent and processed for the
cobblestone area-forming cell (CAFC) assay. (Left) Real-time-polymerase chain reaction
(RT-PCR) analysis of transduced (GFP+) HSPC. (Right top) Representative pictures of
transduced day 7 cobblestones. (Right bottom) Quantification of the frequency of HU-sensitive
CAFC. n=3. (B and C) Epigenetic age predictions were determined based on DNA methylation
at three CpG sites (Prima1, Hsf4, Kcns1). For B6 mice they followed a linear regression
curve, whereas for D2 it followed a logarithmic trend, as previously described.
10
The deviance is the difference of the calculated age and the “real” chronological
age of the four mouse strains. Mice per group: 26-40. (B) Regression curves and (C)
dot plots of the corresponding methylation analyses. *P<0.05; ***P<0.001. PH: phase
contrast; ns: not significant.
Ultimately, the question remains whether the locus also accounts for a variation in
life span. Previously, the methy-lation status of CpG sites within the genes Prima1,
Hsf4, Kcns1 was shown to qualify as a reliable predictor of chronological age of B6
mice.
10
This same study also revealed enhanced epigenetic aging of the D2 strain in accordance
with its general reduced mean life span, supporting the possibility that the panel
might also serve as a marker for the biological age in mice. Applying this B6-trained
marker panel to our (congenic) experimental strains, we observed that epigenetic age
predictions correlated with chronological age in B6 (R2=0.93) and line A mice (R2=0.89).
Notably, epigenetic aging was clearly accelerated in line A mice compared to B6 (Figure
4B and C). We have previously demonstrated that in D2 mice the same epigenetic age
predictor significantly accelerated epigenetic age predictions that rather follow
a logarithmic regression,
10
which, however, line K did not deviate from (Figure 4B and C). More in depth analyses
for line K would warrant the development of an improved age predictor that is adjusted
to more control samples of D2, as the initial marker panel was trained on B6. However,
the data are consistent with a possible role of the QTL in affecting lifespan at least
of line A mice, which will need to be tested in longevity studies of larger cohorts
of animals.
Discussion
Forward genetic approaches in BXD RI strains have been shown to allow for the identification
of QTL linked to lifespan and changes in various tissues and cells upon aging.
22,23
We previously reported the likely linkage of a locus on the distal part of murine
chromosome 11 to two phenotypes, regulation of lifespan as well the susceptibility
of HSPC to short-term treatment with HU. While this finding implies a common mechanism
of regulation for the two phenotypes, speculations on the mechanistic connection between
these two phenotypes remains difficult without the identification of the gene within
the locus regulating at least one of the phenotypes. Here, by generating and analyzing
reciprocal strains congenic for the interval on chromosome 11 (B6 onto D2 and D2 onto
B6), we verify the initial linkage analysis by demonstrating that this locus indeed
controls the susceptibility of HSPC to HU. Other loci than the chromosome 11 locus
may at least in part also contribute to the HU response phenotype, as line A and K
mice are also congenic for other loci in addition to the locus on chromosome 11 (Online
Supplementary Figure S1). The proximal locus on chromosome 11, which spans about 18.6
Mb, is, however, the only region which is identical between both congenic mouse strains,
making a substantial contribution of other loci less likely (Online Supplementary
Table S2). Unexpectedly, elevated sensitivity of HSPC to HU is not linked to altered
cell cycle activity and thus elevated numbers of HSPC in S-phase, nor to apoptosis,
senescence or enhanced replication fork stalling as might be anticipated by previously
reported outcomes to HU exposure. The precise mechanism that confers elevated susceptibility
thus still remains to be further investigated. Our data strongly support Pttg1/Securin
to be the QTL gene in that interval, as elevated levels of its expression conferred
by the D2 allele result in increased HU susceptibility of HSPC. Recently, Pttg1 overexpression
was reported to restrict BrdU incorporation and cause enhanced levels of senescence
and DNA damage in proliferating human fibroblasts,
24
a feature which is not mirrored in HSPC according to our data. Thus, these mechanistic
differences illustrate the unique properties of HSPC with respect to cell cycle regulation
and DNA damage response, as also demonstrated recently.
25–27
The initial linkage data also imply a role for Pttg1 in regulating lifespan. The primary
role of Pttg1 is an inhibition of Separase. This cysteine protease opens cohesin rings
to allow for transition from metaphase to anaphase.
28
Pttg1 is thus seen primarily as a target of the anaphase promoting complex (APC/C)
to initiate chromosome segregation, although other additional roles have been described
in the literature, such as a central role in pituitary tumor formation when over-expressed.
29
Interestingly, the APC/C is directly involved in regulating lifespan in yeast and
results in dysregulation of rDNA biology,
30
while likely dominant negative mutations in cohesin genes have been recently identified
as novel contributors to the initiation of acute myeloid leukemia through modulation
of chromatin accessibility in HSPC and subsequent inhibition of differentiation by
recruiting “stemness” transcription factors to the daughter cells upon division. Extended
presence of cohesin, in the case of elevated levels of Pttg1, might thus contribute
to loss of HSPC potential, which would be consistent with our phenotype (Online Supplementary
Figure S4B). Hence, the two phenotypes might be mechanistically connected via alterations
in the epigenetic landscape rather than changes in chromatid cohesion itself. This
interpretation is supported by the finding that age-associated DNA methylation changes
are acquired at a different pace in congenic mouse strains. It is thus possible that
HU treatment interferes with epigenetic parameters regulated by Pttg1/Securin.
Supplementary Material
Brown et al. Graphical Abstract
Brown et al. Supplementary Appendix
Disclosures and Contributions