Background: Several aspects of herpes simplex virus ICP27 trafficking remain unclear.
Results: We investigated if ICP27 could interact with the nuclear pore complex, finding that ICP27 directly binds the core nucleoporin Nup62.
Conclusion: ICP27 association with Nup62 may provide additional binding sites at the pore for ICP27 shuttling.
Significance: We propose that ICP27 competes with some transport receptors, resulting in inhibition of host pathways and supporting ICP27-mediated transport of HSV-1 mRNAs.
The herpes simplex virus ICP27 protein is important for the expression and nuclear export of viral mRNAs. Although several binding sites have been mapped along the ICP27 sequence for various RNA and protein partners, including the transport receptor TAP of the host cell nuclear transport machinery, several aspects of ICP27 trafficking through the nuclear pore complex remain unclear. We investigated if ICP27 could interact directly with the nuclear pore complex itself, finding that ICP27 directly binds the core nucleoporin Nup62. This is confirmed through co-immunoprecipitation and in vitro binding assays with purified components. Mapping with ICP27 deletion and point mutants further shows that the interaction requires sequences in both the N and C termini of ICP27. Expression of wild type ICP27 protein inhibited both classical, importin α/β-dependent and transportin-dependent nuclear import. In contrast, an ICP27 point mutant that does not interact with Nup62 had no such inhibitory effect. We suggest that ICP27 association with Nup62 provides additional binding sites at the nuclear pore for ICP27 shuttling, thus supporting ICP27-mediated transport. We propose that ICP27 competes with some host cell transport receptors for binding, resulting in inhibition of those host transport pathways.