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Optical Detection of Hairs

International Journal of Trichology

Medknow Publications & Media Pvt Ltd

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      Most cited references 2

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      Investigation of dyed human hair fibres using apertureless near-field scanning optical microscopy.

      We present the first studies of dyed human hair fibres performed with an apertureless scanning near-field optical microscope. Samples consisted of 5-microm-thick cross-sections, the hair fibres being bleached and then dyed before being cut. Hair dyed with two molecular probes diffusing deep inside the fibre or mainly spreading at its periphery were investigated at a wavelength of 655 nm. An optical resolution of about 50 nm was achieved, well below the diffraction limit; the images exhibited different optical contrasts in the cuticle region, depending on the nature of the dye. Our results suggest that the dye that remains confined at the hair periphery is mainly located at its surface and in the endocuticle.
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        Confocal microscopy of hair.

        Confocal microscopy is an excellent method for studying the localization of fluorescent stains. Used in this way, superior 3D images can be obtained from multiple optical sections with very shallow depth of field. The main advantage of this technique is that the sample is not damaged. We have taken serial confocal sections of hair and via specific image enhancement routines have obtained high-quality 3D images enabling the visualization of cuticle scale and its pattern of distribution. This has been done on various types of hair: bleached, permed and in certain pathological conditions. This first step will allow us to characterize the hair surface in terms of its roughness, and the distribution and form of cuticular scale, parameters that have potential in the assessment of dermocosmetic efficacy.
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          Author and article information

          Affiliations
          Departments of Periodontology and Oral Implantology, Rural Dental College, Loni, Maharashtra, India
          Author notes
          Address for correspondence: Dr. Rajiv Saini, Departments of Periodontology and Oral Implantology, Rural Dental College, Loni, Rahata, Ahmednagar, Maharashtra - 413 736, India. E-mail: drperiodontist@ 123456yahoo.co.in
          Journal
          Int J Trichology
          IJT
          International Journal of Trichology
          Medknow Publications & Media Pvt Ltd (India )
          0974-7753
          0974-9241
          Jul-Dec 2011
          : 3
          : 2
          : 128-129
          3250015
          22223982
          IJT-3-128
          10.4103/0974-7753.90849
          Copyright: © International Journal of Trichology

          This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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          Dermatology

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