Glycoprotein: Glycosyl Transferase Activities of Rat Kidney Plasma Membranes

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Abstract

Rat kidney plasma membrane preparations and suspensions of intact glomeruli were used to study glycoprotein: glycosyl transferase activities present on cell surfaces. Collagen: glucosyl, fetuin: galactosyl, and fetuin: sialic acid (sialíc acid is the generic term for N-acetylneuraminic acid, NANA) transferase activities were increased from 1.9- to 5.6-fold in membrane preparations compared to whole tissue homogenates. Considerable activity was found with suspensions of glomeruli though in each assay specific activity was substantially less than for glomeruli homogenized and sonicated in buffer containing the nonionic detergent Triton X-100. For both membranes and glomeruli, collagen: glucosyl and fetuin: galactosyl transferase activities were enhanced by addition of folic acid whereas sialic acid transferase activity was reduced.

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Journal

Journal ID (publisher-id): NEF

Journal ID (nlm-ta): Nephron

Journal ID (doi): 10.1159/issn.1660-8151

Title: Nephron

Publisher: S. Karger AG

ISSN (Print): 1660-8151

ISSN (Electronic): 2235-3186

Publication date Subscription-year: 1974

Publication date (Print): 1974

Publication date (Electronic): 28 November 2008

Volume: 12

Issue: 3

Pages: 211-218

Affiliations

Department of Pharmacology and Toxicology, University of Rochester School of Medicine and Dentistry, Rochester, N.Y

Article

Publisher ID: 180377 Other ID: Nephron 1974;12:211–218

DOI: 10.1159/000180377

PubMed ID: 4135061

SO-VID: 074bb68c-3e1e-46b6-bc8d-5eaa984bcd69

License:

Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

History

Date received : 07 June 1973

Date accepted : 30 July 1973

Page count

Pages: 8

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