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      Febrile urinary tract infection in an immunocompetent male caused by Burkholderia multivorans- evidence of transmission in a home hot tub

      case-report
      a , b , * , a , c
      IDCases
      Elsevier
      Burkholderia, Urinary tract infection

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          Abstract

          Burkholderia are opportunistic pathogens. Here we describe a case of Burkholderia multivorans urinary tract infection in a previously healthy man. The bacterium was likely introduced during sexual intercourse in a home hot tub contaminated with the bacterium. The infection led to urinary retention and was successfully treated with trimethoprim-sulfamethoxazole.

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          Nosocomial Burkholderia cepacia infections in a Turkish university hospital: a five-year surveillance.

          Burkholderia cepacia has the potential to cause fatal infections in ICUs, and multidrug resistance makes them a serious threat in hospital settings. The aim of this study was to evaluate the epidemiology of B. cepacia infections in our hospital. The incidence, clinical characteristics, antimicrobial susceptibility, and outcomes of nosocomial B. cepacia infections during a five-year period were retrospectively analysed according to the infection control committee records. A total of 39 cases with nosocomial B. cepacia infection were included in the study. B. cepacia was identified from 0.7% of the nosocomial isolates. Its incidence was 0.26 per 1,000 admissions with 53.8% crude mortality rate. The most frequent nosocomial B. cepacia infection was pneumonia (58.9%), followed by bloodstream infections (25.6%), surgical site infections (7.6%), urinary tract infections, (5.1%), and skin-soft tissue infections (2.5%). Nosocomial B. cepacia infections were most commonly observed in intensive care units (61.5%). The most active antimicrobial agents were piperacillin-tazobactam, cefoperazone-sulbactam, and carbapenems. The incidence of nosocomial B. cepacia infections was rare in our hospital, and no outbreak was detected during the study period. However, infections caused by B. cepacia should be taken into consideration because of their high mortality due to multidrug resistance in ICU settings.
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            Application of a recA gene-based identification approach to the maize rhizosphere reveals novel diversity in Burkholderia species.

            Burkholderia species are widely distributed in the natural environment. We evaluated the use of the recA gene in a cultivation-independent approach to examine the Burkholderia diversity associated with the maize rhizosphere. Two types of recA gene library were constructed, one with broad-specificity recA primers (BUR1 and BUR2) and a second from the products of nested PCRs using Burkholderia-specific primers (BUR3 and BUR4). The broad-specificity primer set provided near full-length recA sequences (869 bp) suitable for the creation of robust environmental sequence data sets; however, the nested PCR approach demonstrated the greatest specificity (84%) for detection of Burkholderia species recA genes. In addition, the screening approach was able to identify recA phylotypes matching Burkholderia cepacia complex species previously cultivated from the maize samples and discriminate these from other Burkholderia. The ecological benefit of Burkholderia species cultivated from maize rhizosphere is well documented, however, the fact that the majority of Burkholderia recA genes detected in this study (90%) were suggestive of novel taxa indicates that a wealth of potentially important interactions with uncultivated Burkholderia species remain unstudied in this habitat.
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              First microbiota assessments of children's paddling pool waters evaluated using 16S rRNA gene-based metagenome analysis

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                Author and article information

                Contributors
                Journal
                IDCases
                IDCases
                IDCases
                Elsevier
                2214-2509
                03 May 2019
                2019
                03 May 2019
                : 17
                : e00553
                Affiliations
                [a ]Lund University, Medical Faculty, Department for Clinical Sciences Lund, Division of Infection Medicine, Lund, Sweden
                [b ]Skåne University Hospital, Division for Infectious Diseases, Lund, Sweden
                [c ]Clinical Microbiology, Laboratory Medicine, Region Skåne, Lund, Sweden
                Author notes
                [* ]Corresponding author at: Division of Infection Medicine Diseases, Department of Clinical Sciences Lund, Lund University, BMC B14, SE-223 63, Lund, Sweden. magnus.rasmussen@ 123456med.lu.se
                Article
                S2214-2509(19)30105-2 e00553
                10.1016/j.idcr.2019.e00553
                6510961
                31110946
                08191d31-15bc-4b9a-8bea-f9f9581f5706
                © 2019 The Authors

                This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

                History
                : 29 April 2019
                : 1 May 2019
                : 1 May 2019
                Categories
                Article

                burkholderia,urinary tract infection
                burkholderia, urinary tract infection

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