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      Immunofluorescence Studies of Chick Lens FISC and α-Crystallin Antigens during Lens Morphogenesis and Development

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          Abstract

          Indirect staining method of Weller and Coons [23] was applied to detect the presence of FISC and α-crystallin lens antigens in the embryonic and post-embryonic chick lenses. Specific antiserum against these 2 antigens was prepared by a method of immunological tolerance in new born rabbits. Earliest reactions against either of the 2 antisera were not restricted to few cells only as has been reported earlier. The central non-nucleated fiber area, the so-called lens nucleus, from a 6-week-old chick lens gave negative immuno-fluorescence reactions against both the antisera, but presence of these 2 antigens in the nuclear region could be detected by immuno-electrophoresis

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          Author and article information

          Journal
          ORE
          Ophthalmic Res
          10.1159/issn.0030-3747
          Ophthalmic Research
          S. Karger AG
          0030-3747
          1423-0259
          1971
          1971
          27 November 2009
          : 2
          : 6
          : 344-357
          Affiliations
          Department of Medical Anatomy and Embryology, The State University, Janskerkhof 3A, Utrecht, The Netherlands
          Article
          264584 Ophthal Res 1971;2:344–357
          10.1159/000264584
          © 1971 S. Karger AG, Basel

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          Pages: 14
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