B. Wilde , A. Mertens , S. J. Arends , R. P. Rouhl , R. Bijleveld , J. Huitema , S. A. Timmermans , J. Damoiseaux , O. Witzke , A. M. Duijvestijn , P. van Paassen , R. J. van Oostenbrugge , J. W. Cohen Tervaert
23 June 2016
Endothelial progenitor cells (EPC) are of major importance in vascular repair under healthy circumstances. Vascular injury in need of repair occurs frequently in ANCA-associated vasculitis (AAV). A specialized T cell subset enhancing EPC function and differentiation has recently been described. These angiogenic T cells (T ang) may have an important impact on the vascular repair process. Therefore, the aim of our study was to investigate EPC and T ang in AAV.
Fifty-three patients suffering from AAV and 29 healthy controls (HC) were enrolled in our study. Forty-four patients were in remission, nine patients were in active state of disease. Patients were either untreated or were under monotherapy with low-dose steroids (max. 5 mg/day) at the time of sampling. Circulating EPC and T ang were determined by flow cytometry (FACS). The functional capacity of EPC was assessed by established cell culture methods.
Circulating EPC were significantly decreased in AAV as compared to HC. The capacity of EPC to differentiate and proliferate was differentially impaired in patients as compared to HC. The outgrowth of endothelial colony-forming cells (ECFC) was severely decreased in patients whereas colony-forming units-endothelial cell (CFU-EC) outgrowth was unaffected. ECFC and CFU-EC differentiation was strictly T cell-dependent. Patients with a relapsing disease course had an impaired ECFC outgrowth and expansion of T ang as compared to patients with a stable, nonrelapsing disease.