Blog
About

0
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found

      Effects of Tumor Necrosis Factor on Glomerular Mesangial and Epithelial Cells in Culture

      , ,

      Nephron

      S. Karger AG

      Glomerulonephritis, Cytokine

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          We have previously documented the importance of tumor necrosis factor (TNF) alpha in the pathogenesis of nephrotoxic-serum nephritis in rats. In this study, we evaluated the possible relevance of the well-established cytocidal TNF activity to the mechanism of glomerular injury by assessing sensitivity of rat mesangial and epithelial cell populations to recombinant murine TNF alpha (rMuTNF). Radiolabelled confluent mesangial cell cultures that were incubated with rMuTNF released significantly more <sup>3</sup>H-thymidine than control monolayers (maximum specific release was 11.4 ± 4.9% at 1,000 pg/ml of rMuTNF). rMuTNF was, however, approximately 1,000-fold less cytolytic in mesangial cells than in the lymphotoxin-sensitive L929 cell line. Conversely, glomerular epithelial cells were not affected by exposure to rMuTNF under the same conditions. These results suggest that the cytolytic effect of TNF may contribute to glomerular injury in nephrotoxic-serum nephritis.

          Related collections

          Author and article information

          Journal
          NEF
          Nephron
          10.1159/issn.1660-8151
          Nephron
          S. Karger AG
          1660-8151
          2235-3186
          1990
          1990
          10 December 2008
          : 56
          : 4
          : 410-413
          Affiliations
          Department of Medicine, Royal Victoria Hospital, McGill University, Montreal, Canada
          Article
          186185 Nephron 1990;56:410–413
          10.1159/000186185
          2080000
          © 1990 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 4
          Categories
          Original Paper

          Cardiovascular Medicine, Nephrology

          Glomerulonephritis, Cytokine

          Comments

          Comment on this article