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      Characterization of ribosomal DNA (rDNA) in Drosophila arizonae

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          Abstract

          Ribosomal DNA (rDNA) is a multigenic family composed of one or more clusters of repeating units (RU). Each unit consists of highly conserved sequences codifying 18S, 5.8S and 28S rRNA genes intercalated with poorly conserved regulatory sequences between species. In this work, we analyzed the rDNA of Drosophila arizonae, a member of the mulleri complex (Repleta group). Using genomic restriction patterns, cloning and mapping of some representative rDNA fragments, we were able to construct a representative restriction map. RU in this species are 13.5-14 kb long, restriction sites are completely conserved compared with other drosophilids and the rDNA has an R1 retrotransposable element in some RU. We were unable to detect R2 elements in this species.

          Translated abstract

          O DNA ribossômico (rDNA) é uma família multigênica composta de um ou mais aglomerados de unidades de repetição (RU). Cada unidade consiste de seqüências altamente conservadas que codificam os rRNAs 18S, 5.8S e 28S, intercaladas com seqüências regulatórias pouco conservadas entre as espécies. Neste trabalho analisamos o rDNA de Drosophila arizonae, um membro do complexo mulleri (grupo Repleta). Usando padrões de restrição genômicos, clonagem e mapeamento de alguns fragmentos de rDNA representativos, estabelecemos um mapa de restrição do rDNA representativo desta espécie. Neste drosofilídeo, a RU tem um tamanho médio de 13.5-14 kb e os sítios de restrição estão completamente conservados com relação a outras drosófilas. Além disto, este rDNA possui um elemento transponível tipo R1 presente em algumas unidades. Neste trabalho não tivemos evidências da presença de elementos R2 no rDNA desta espécie.

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          Most cited references9

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          Molecular Cloning : A Laboratory Manual

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            Drosophila, a laboratory manual

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              Further observations on the nucleolar organizing activity in salivary gland cells of Drosophila mulleri, D. arizonensis and their hybrids

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                gmb
                Genetics and Molecular Biology
                Genet. Mol. Biol.
                Sociedade Brasileira de Genética (Ribeirão Preto )
                1678-4685
                June 2000
                : 23
                : 2
                : 231-233
                Affiliations
                [1 ] Universidade Federal do Rio de Janeiro Brazil
                [2 ] Universidade Federal de Alagoas Brasil
                Article
                S1415-47572000000200015
                10.1590/S1415-47572000000200015
                08696725-62b4-433a-b595-cb253955f934

                http://creativecommons.org/licenses/by/4.0/

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                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=1415-4757&lng=en
                Categories
                BIOCHEMISTRY & MOLECULAR BIOLOGY
                GENETICS & HEREDITY

                Molecular biology,Genetics
                Molecular biology, Genetics

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