We investigated the relationship of implicit racial prejudice to discriminatory behavior. White university students chose the best of three applicants (two were White and one was Black) for a prestigious teaching fellowship. They then completed the Implicit Association Test (IAT), a measure of implicit racial bias. Three weeks later, participants completed a second implicit measure of racial bias by viewing photos of Whites and Blacks while facial electromyography (EMG) was recorded from sites corresponding to the muscles used in smiling and frowning. Analyses revealed that bias in cheek EMG activity was related to the race of the chosen applicant, whereas bias on the IAT was not. Motivations to control prejudiced reactions were not related to EMG activity or the race of the applicant chosen, but were related to IAT bias. The findings indicate that facial EMG can be used as an implicit measure of prejudice related to discrimination.