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      Proposta para teste carrapaticida por imersão de larvas de Rhipicephalus (Boophilus) microplus: avaliação em cipermetrina e amitraz Translated title: Proposal of an acaricide laboratory test larvae immersion of Rhipicephalus (Boophilus) microplus: evaluation with cypermethrin and amitraz

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          Abstract

          Objetivou-se desenvolver uma técnica de avaliação de carrapaticidas por imersão de larvas de Riphicephalus (Boophilus) microplus. As larvas foram obtidas a partir de ovos de 30 teleóginas coletadas em bovinos de uma propriedade rural do município de Lages, SC, Brasil, os quais foram acondicionados em alíquotas de 10mg por seringa descartável de 5ml, previamente preparadas e mantidas em câmara climatizada até a eclosão. Larvas com sete a dez dias de idade foram submetidas à imersão por 30 segundos em oito diluições de produtos carrapaticidas comerciais à base de amitraz e de cipermetrina, cada uma com dez repetições, sendo mantido um grupo controle para cada repetição e produto, o qual foi imerso em água destilada. A leitura foi realizada 24 horas após o tratamento com a contagem de larvas vivas e mortas. A média de DL50 para cipermetrina foi de 2,99ppm e o erro padrão de 0,36 e para o amitraz foi de 17,22ppm e o erro padrão de 4,20, com intervalo de confiança (IC) 95%.

          Translated abstract

          The aim of this work was to develop a technique of acaricides evaluation by immersion of larvae. Larvae were obtained from 30 engorged females of Riphicephalus (Boophilus) microplus were collected in a ranch in Lages, Santa Catarina state, Brazil. Larvae were obtained from 10mg of eggs placed into 5ml disposable syringes, previously prepared and kept under controlled conditions of laboratory, and collected between the third and the twelfth day of oviposition. Seven to ten days old larvae were submitted to the acaricide laboratory test, in ten replications, by their immersion in eight dilutions of commercial acaricides (amitraz or cypermethrin), for 30 seconds; for each replication and commercial product, a control group was immersed in distilled water. Results were evaluated 24 hours after each treatment by counting the number of live and dead larvae. The mean LD50 values and standard error of the mean for cypermethrin and amitraz were 2.99 ± 0.36 and 17.22 ± 4.20 ppm, respectively, for a confidence interval of 95%. The proposed acaricide laboratory test procedures by larvae immersion offered promising results for the evaluation of acaricides.

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          Most cited references 6

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          Culture of an organophosphorus-resistant strain of Boophilus microplus (Can.) and an assessment of its resistance spectrum.

           R. D. Shaw (1966)
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            Avaliação in vitro de uma cepa de campo de Boophilus microplus (Acari: Ixodidae) resistente à Amitraz

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              • Record: found
              • Abstract: not found
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              Método para avaliação de mortalidade de larvas de Boophilus microplus (CANESTRINI, 1887) submetidas a tratamentos com produtos carrapaticidas

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                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Role: ND
                Journal
                rbpv
                Revista Brasileira de Parasitologia Veterinária
                Rev. Bras. Parasitol. Vet.
                Colégio Brasileiro de Parasitologia Veterinária (Jaboticabal )
                1984-2961
                December 2008
                : 17
                : 4
                : 242-245
                Affiliations
                [1 ] Universidade do Estado de Santa Catarina Brazil
                [2 ] Universidade do Estado de Santa Catarina Brazil
                [3 ] Universidade do Estado de Santa Catarina Brazil
                S1984-29612008000400014
                10.1590/S1984-29612008000400014

                http://creativecommons.org/licenses/by/4.0/

                Product
                Product Information: SciELO Brazil
                Categories
                PARASITOLOGY
                VETERINARY SCIENCES

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