16
views
0
recommends
+1 Recommend
1 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: found
      Is Open Access

      Maintenance media for the axolotl Ambystoma mexicanum juveniles (Amphibia: Caudata) Translated title: Soluciones de mantenimiento de juveniles del ajolote Ambystoma mexicanum (Amphibia: Caudata)

      research-article

      Read this article at

      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Physiological condition and organisms' health which are grown in culture systems depends on several factors including water quality, feeding and density among others. In Mexico, the colonies of the axolotl Ambystoma mexicanum (Shaw), an indigenous amphibian under extinction risk, are maintained under different culture conditions according to the objectives of the colony and the available resources. Particularly, water electrolytic characteristic and ionic and osmotic conditions are the factors with greater variation in the axolotl culture systems. Therefore, it is necessary to standardize the best maintenance conditions to store the germoplasm of the axolotl and to ensure healthy organisms with researching purposes. Thus, the aim of the present study was to evaluate the development and growth of Ambystoma mexicanum larvae reared under different maintenance media, usually used in Mexico for the culture of the species: 1) dechlorinated tap water; 2) dechlorinated tap water enriched with sodium chloride and commercial colloidal solution and 3) Holtfreter's solution reconstructed with dechlorinated tap water. In each experimental condition, 15 larvae on stage 44 (immediately after hatching) were maintained during 21 days and development and growth were weekly recorded. Ionic and osmotic conditions of the external media were routinely registered. The obtained results suggested a better physiological condition of the axolotls maintained on Holtfreter's solution, where the highest growth rate (13 g WW d-1) and the greatest condition factor (0.79) were registered. The use of this solution is recommended due it guarantees the suitable development of early stages of A. mexicanum on culture systems.

          Translated abstract

          La condición fisiológica y por lo tanto la salud de los organismos acuáticos depende de varios factores como la calidad del agua de mantenimiento, la alimentación, la densidad, entre otros. En México, las colonias del ajolote Ambystoma mexicanum (Shaw), anfibio endémico y en riesgo de extinción en su hábitat, son mantenidas bajo diferentes condiciones de cultivo de acuerdo con los objetivos de la colonia y los recursos disponibles. Particularmente, las características electrolíticas del agua de mantenimiento y las condiciones iónicas y osmóticas, son los factores que presentan mayor variación entre los diferentes sistemas de cultivo. Por lo tanto, se considera necesario estandarizar las condiciones adecuadas de mantenimiento de los ajolotes para mantener el germoplasma de la especie y para garantizar organismos sanos con fines de investigación. El objetivo del presente estudio fue evaluar el desarrollo y el crecimiento de larvas del ajolote en tres diferentes medios usualmente utilizados en México en el mantenimiento de las colonias del ajolote: agua de la llave libre de cloro, agua de la llave con solución comercial salina y de coloides y solución Holtfreter. Los resultados obtenidos denotan una mejor condición fisiológica de los ajolotes mantenidos en la solución Holtfreter, medio en el cuál registraron la mayor tasa de crecimiento (13 g WW d-1) y un mayor índice de condición (0.79). De acuerdo con estos resultados se propone el uso de la solución Holtfreter para garantizar el adecuado desarrollo de los estadios tempranos de A. mexicanum bajo condiciones de mantenimiento y/o cultivo controlado.

          Related collections

          Most cited references30

          • Record: found
          • Abstract: not found
          • Article: not found

          Morphometric criteria for estimating sexual maturity in two snappers, Etelis carbunculus and Pristipomoides sieboldii

            Bookmark
            • Record: found
            • Abstract: found
            • Article: not found

            The role of thyroid hormone in zebrafish and axolotl development.

            Exogenous thyroid hormone (TH) induces premature differentiation of the zebrafish pectoral fins, which are analogous to the forelimbs of tetrapods. It accelerates the growth of the pelvic fins but not precociously. Goitrogens, which are chemical inhibitors of TH synthesis by the thyroid gland, inhibit the transition from larva to juvenile fish including the formation of scales, and pigment pattern; they stunt the growth of both pectoral and pelvic paired fins. Inhibition by goitrogens is rescued by the simultaneous addition of thyroxine. The effect of adding TH to the rearing water of the postembryonic Mexican axolotl was reinvestigated under conditions that permit continued growth and development. In addition to morphological changes that have been described, TH greatly stimulates axolotl limb growth causing the resulting larva to be proportioned as an adult in about two months. This study extends the known evolutionary relatedness of tetrapod limbs and fish fins to include the TH stimulation of salamander limb and zebrafish fin growth, and suggests that TH is required to complete the life cycle of a typical bony fish and a salamander at the same developmental stage that it controls anuran and flounder metamorphosis.
              Bookmark
              • Record: found
              • Abstract: found
              • Article: not found

              Regeneration of the urodele limb: a review.

              Urodele amphibians have been widely used for studies of limb regeneration. In this article, we review studies on blastema cell proliferation and propose a model of blastemal self-organization and patterning. The model is based on local cell interactions that intercalate positional identities within circumferential and proximodistal boundaries that outline the regenerate. The positional identities created by the intercalation process appear to be reflected in the molecular composition of the cell surface. Transcription factors and signaling molecules involved in patterning are discussed within the context of the boundary/intercalation model. Copyright 2003 Wiley-Liss, Inc.
                Bookmark

                Author and article information

                Contributors
                Role: ND
                Role: ND
                Role: ND
                Journal
                hbio
                Hidrobiológica
                Hidrobiológica
                UAM, Unidad Iztapalapa, (México )
                0188-8897
                December 2009
                : 19
                : 3
                : 205-210
                Affiliations
                [1 ] Universidad Nacional Autónoma de México Mexico
                [2 ] Universidad Nacional Autónoma de México Mexico
                Article
                S0188-88972009000300003
                090857cc-75c6-4af7-b4ee-8f5f05e73eff

                http://creativecommons.org/licenses/by/4.0/

                History
                Categories
                Marine & Freshwater Biology

                Ecology
                Ambystoma mexicanum,maintenance,development,growth,Holtfreter's solution,mantenimiento,desarrollo,crecimiento,solución de Holtfreter

                Comments

                Comment on this article