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      Ribosomal DNA sequencing for identification of aerobic gram-positive rods in the clinical laboratory (an 18-month evaluation).

      Journal of Clinical Microbiology
      Bacteria, Aerobic, genetics, isolation & purification, DNA, Bacterial, chemistry, DNA, Ribosomal, Gram-Positive Rods, Humans, Phenotype, Prospective Studies, RNA, Ribosomal, 16S, Sequence Analysis, DNA

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          Abstract

          We have evaluated over a period of 18 months the use of 16S ribosomal DNA (rDNA) sequence analysis as a means of identifying aerobic gram-positive rods in the clinical laboratory. Two collections of strains were studied: (i) 37 clinical strains of gram-positive rods well identified by phenotypic tests, and (ii) 136 clinical isolates difficult to identify by standard microbiological investigations, i.e., identification at the species level was impossible. Results of molecular analyses were compared with those of conventional phenotypic identification procedures. Good overall agreement between phenotypic and molecular identification procedures was found for the collection of 37 clinical strains well identified by conventional means. For the 136 clinical strains which were difficult to identify by standard microbiological investigations, phenotypic characterization identified 71 of 136 (52.2%) isolates at the genus level; 65 of 136 (47.8%) isolates could not be discriminated at any taxonomic level. In comparison, 16S rDNA sequencing identified 89 of 136 (65.4%) isolates at the species level, 43 of 136 (31.6%) isolates at the genus level, and 4 of 136 (2.9%) isolates at the family level. We conclude that (i) rDNA sequencing is an effective means for the identification of aerobic gram-positive rods which are difficult to identify by conventional techniques, and (ii) molecular identification procedures are not required for isolates well identified by phenotypic investigations.

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          Author and article information

          Journal
          12958237
          193817
          10.1128/JCM.41.9.4134-4140.2003

          Chemistry
          Bacteria, Aerobic,genetics,isolation & purification,DNA, Bacterial,chemistry,DNA, Ribosomal,Gram-Positive Rods,Humans,Phenotype,Prospective Studies,RNA, Ribosomal, 16S,Sequence Analysis, DNA

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