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      Recurrent Herpetic Keratitis: Failure to Detect Herpes simplex Virus Infection Using the Syva MicroTrak TM HSV1/HSV2 Direct Specimen Identification/Typing Test

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          A 35-year-old man had developed recurrent herpetic keratitis characterized by dendritic keratitis at intervals of a year. We were able to culture cytopathic agents repeatedly from his lesions by inoculating Vero cells. The cultures yielded definitive evidence of a virus that caused a cytopathic effect within 3 days. However, these virus strains could not be identified as herpes simplex virus (HSV) in immunofluorescence assays using the Syva MicroTrak<sup>TM</sup> HSV1/HSV2 direct specimen identification/typing test. Rather they were identified as strains of HSV type 1 (HSV-1) on the basis of plaque morphology, neutralization tests, electron-microscopic examination and DNA restriction endonuclease analysis. Our results allow us to assume the existence of HSV-1 strains isolated clinically that are negative to analysis using the Syva MicroTrak<sup>TM</sup> HSV1/HSV2 direct specimen identification/typing test.

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          S. Karger AG
          31 March 2010
          : 201
          : 4
          : 169-173
          Departments of aOphthalmology and bVirology, Faculty of Medicine, Kyushu University, Fukuoka, Japan
          310147 Ophthalmologica 1990;201:169–173
          © 1990 S. Karger AG, Basel

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