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Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance.

Author(s): I U S Leong, R Karjalainen, A Ellingboe, T Tooley, Ivanhoe K H Leung, U Lehtinen, Marilyn D. Skinner

Publication date: 1990-04-30

Keywords: Anti-Bacterial Agents, pharmacology, Ascomycota, drug effects, genetics, Blotting, Southern, Cosmids, Drug Resistance, Microbial, Hygromycin B, Plasmids, Transformation, Genetic

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Abstract

Low frequency, integrative transformation of three fertile hermaphroditic strains of Magnaporthe grisea has been achieved using plasmid pAN7-1 and cosmid pAN7-2, which contain an Escherichia coli hygromycin B phosphotransferase gene linked to Aspergillus nidulans regulatory sequences.

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PubMed ID:: 2357737

ScienceOpen disciplines: Chemistry

Keywords: Anti-Bacterial Agents,pharmacology,Ascomycota,drug effects,genetics,Blotting, Southern,Cosmids,Drug Resistance, Microbial,Hygromycin B,Plasmids,Transformation, Genetic

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ScienceOpen disciplines: Chemistry

Keywords: Anti-Bacterial Agents, pharmacology, Ascomycota, drug effects, genetics, Blotting, Southern, Cosmids, Drug Resistance, Microbial, Hygromycin B, Plasmids, Transformation, Genetic

Transformation of the rice blast fungus Magnaporthe grisea to hygromycin B resistance.

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