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In vitro evolution and analysis of HIV-1 LTR-specific recombinases
Author(s):
Frank Buchholz
,
Joachim Hauber
Publication date
Created:
January 2011
Publication date
(Print):
January 2011
Journal:
Methods
Publisher:
Elsevier BV
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There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.
Abstract
Current antiretroviral therapies would greatly benefit from the concurrent removal of integrated HIV-1 proviral DNA from the patient's cells. In this review, we describe an experimental strategy that allowed the engineering and functional analysis of a HIV-1 LTR-specific recombinase (Tre-recombinase). We furthermore provide protocols that are utilized for the investigation of Tre's antiretroviral activity in infected tissue cultures as well as in infected humanized Rag2(-/-)γc(-/-) mice. Copyright © 2010 Elsevier Inc. All rights reserved.
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Crocodylian evolution
Author and article information
Journal
Title:
Methods
Abbreviated Title:
Methods
Publisher:
Elsevier BV
ISSN (Print):
10462023
Publication date Created:
January 2011
Publication date (Print):
January 2011
Volume
: 53
Issue
: 1
Pages
: 102-109
Article
DOI:
10.1016/j.ymeth.2010.06.014
PubMed ID:
20600935
SO-VID:
0a12ca4f-bcfb-4afe-8c98-da05d467387a
Copyright ©
© 2011
License:
https://www.elsevier.com/tdm/userlicense/1.0/
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