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      Microfluidic single-cell real-time PCR for comparative analysis of gene expression patterns.

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          Abstract

          Single-cell quantitative real-time PCR (qRT-PCR) combined with high-throughput arrays allows the analysis of gene expression profiles at a molecular level in approximately 11 h after cell sample collection. We present here a high-content microfluidic real-time platform as a powerful tool for comparatively investigating the regulation of developmental processes in single cells. This approach overcomes the limitations involving heterogeneous cell populations and sample amounts, and may shed light on differential regulation of gene expression in normal versus disease-related contexts. Furthermore, high-throughput single-cell qRT-PCR provides a standardized, comparative assay for in-depth analysis of the mechanisms underlying human pluripotent stem cell self-renewal and differentiation.

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          Author and article information

          Journal
          Nat Protoc
          Nature protocols
          Springer Science and Business Media LLC
          1750-2799
          1750-2799
          Apr 05 2012
          : 7
          : 5
          Affiliations
          [1 ] Department of Medicine, Stanford University School of Medicine, California, USA.
          Article
          nprot.2012.021 NIHMS456936
          10.1038/nprot.2012.021
          3657501
          22481529
          0a5df376-b2e3-4034-a4e6-0970deb5c8e0
          History

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