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      Three-dimensional structural analysis of eukaryotic flagella/cilia by electron cryo-tomography

      a , b , a , b , a , b , *

      Journal of Synchrotron Radiation

      International Union of Crystallography

      3rd International Symposium on Diffraction Structural Biology (ISDSB2010)

      dynein, flagella, axoneme, tomography, cryo-EM

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          Abstract

          Based on the molecular architecture revealed by electron cryo-tomography, the mechanism of the bending motion of eukaryotic flagella/cilia is discussed.

          Abstract

          Electron cryo-tomography is a potential approach to analyzing the three-dimensional conformation of frozen hydrated biological macromolecules using electron microscopy. Since projections of each individual object illuminated from different orientations are merged, electron tomography is capable of structural analysis of such heterogeneous environments as in vivo or with polymorphism, although radiation damage and the missing wedge are severe problems. Here, recent results on the structure of eukaryotic flagella, which is an ATP-driven bending organelle, from green algae Chlamydomonas are presented. Tomographic analysis reveals asymmetric molecular arrangements, especially that of the dynein motor proteins, in flagella, giving insight into the mechanism of planar asymmetric bending motion. Methodological challenges to obtaining higher-resolution structures from this technique are also discussed.

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          Author and article information

          Conference
          J Synchrotron Radiat
          J. Synchrotron Rad.
          Journal of Synchrotron Radiation
          International Union of Crystallography
          0909-0495
          1600-5775
          01 January 2011
          05 November 2010
          05 November 2010
          : 18
          : Pt 1 ( publisher-idID: s110100 )
          : 2-5
          Affiliations
          [a ]Paul Scherrer Institute, 5232 Villigen PSI, Switzerland
          [b ]ETH Zurich, Switzerland
          Author notes
          Correspondence e-mail: takashi.ishikawa@ 123456psi.ch
          Article
          ys5051 JSYRES S0909049510036812
          10.1107/S0909049510036812
          3004243
          21169680
          © Khanh Huy Bui et al. 2011

          This is an open-access article distributed under the terms of the Creative Commons Attribution Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.

          3rd International Symposium on Diffraction Structural Biology (ISDSB2010)
          Categories
          Diffraction Structural Biology

          Radiology & Imaging

          tomography, dynein, axoneme, flagella, cryo-em

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