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      Highly sensitive and accurate detection of Vibrio cholera O1 OmpW gene by fluorescence DNA biosensor based on gold and magnetic nanoparticles

      , , , , ,
      Process Biochemistry
      Elsevier BV

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          Gold nanoparticles in chemical and biological sensing.

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            Rapid and quantitative detection of human adenovirus DNA by real-time PCR.

            Rapid diagnosis of human adenovirus (HAdV) infections was achieved by PCR in the recent years. However, conventional PCR has the risk of carry-over contamination due to open handling with its products, and results are only qualitative. Therefore, a quantitative "real-time" PCR with consensus primer and probe (dual fluorescence labelled, "TaqMan") sequences for a conserved region of the hexon gene was designed and evaluated. Real-time PCR detected all 51 HAdV prototypes. Sensitivity of the assay was
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              DNA biosensors and microarrays.

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                Author and article information

                Journal
                Process Biochemistry
                Process Biochemistry
                Elsevier BV
                13595113
                February 2018
                February 2018
                : 65
                : 46-54
                Article
                10.1016/j.procbio.2017.10.009
                0ab4d7c4-c026-447a-823e-1cd7c66bb04b
                © 2018

                https://www.elsevier.com/tdm/userlicense/1.0/

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