10
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      The role of vaccinia termination factor and cis-acting elements in vaccinia virus early gene transcription termination.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Vaccinia virus early gene transcription termination requires the virion form of the viral RNA polymerase (vRNAP), Nucleoside Triphosphate Phosphohydrolase I (NPHI), ATP, the vaccinia termination factor (VTF), and a U5NU termination signal in the nascent transcript. VTF, also the viral mRNA capping enzyme, binds U5NU, and NPHI hydrolyzes ATP to release the transcript. NPHI can release transcripts independent of VTF and U5NU if vRNAP is not actively elongating. However, VTF and U5NU are required for transcript release from an elongating vRNAP, suggesting that the function of VTF and U5NU may be to stall the polymerase. Here we demonstrate that VTF inhibits transcription elongation by enhancing vRNAP pausing. Hence VTF provides the connection between the termination signal in the RNA transcript and viral RNA polymerase to initiate transcription termination. We also provide evidence that a second cis-acting element downstream of U5NU influences the location and efficiency of early gene transcription termination.

          Related collections

          Author and article information

          Journal
          Virology
          Virology
          Elsevier BV
          1096-0341
          0042-6822
          Nov 2015
          : 485
          Affiliations
          [1 ] Department of Biological Sciences, University at Buffalo, Buffalo, NY 14260, USA.
          [2 ] Department of Biological Sciences, University at Buffalo, Buffalo, NY 14260, USA. Electronic address: gollinck@buffalo.edu.
          Article
          S0042-6822(15)00254-8
          10.1016/j.virol.2015.05.008
          26280468
          0acd9c66-f8c3-4ff9-9948-2ccd104cc702
          History

          Capping enzyme,RNA polymerase,RNA structure,Transcription factors,Transcription termination,Viral transcription

          Comments

          Comment on this article