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      Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans.

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          Abstract

          A key challenge of functional genomics today is to generate well-annotated data sets that can be interpreted across different platforms and technologies. Large-scale functional genomics data often fail to connect to standard experimental approaches of gene characterization in individual laboratories. Furthermore, a lack of universal annotation standards for phenotypic data sets makes it difficult to compare different screening approaches. Here we address this problem in a screen designed to identify all genes required for the first two rounds of cell division in the Caenorhabditis elegans embryo. We used RNA-mediated interference to target 98% of all genes predicted in the C. elegans genome in combination with differential interference contrast time-lapse microscopy. Through systematic annotation of the resulting movies, we developed a phenotypic profiling system, which shows high correlation with cellular processes and biochemical pathways, thus enabling us to predict new functions for previously uncharacterized genes.

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          Author and article information

          Journal
          Nature
          Nature
          Springer Science and Business Media LLC
          1476-4687
          0028-0836
          Mar 24 2005
          : 434
          : 7032
          Affiliations
          [1 ] Cenix BioScience GmbH, Tatzberg 47-51, D-01307 Dresden, Germany. soennichsen@cenix-bioscience.com
          Article
          nature03353
          10.1038/nature03353
          15791247
          0ad15232-6ab1-46d8-a077-322184cb2984
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