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      Antioxidant capacity in tilapia fillets enriched with extract of acerola fruit residue

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          Abstract

          This work evaluated the effect of supplementation with ethanolic extract of acerola fruit residue (EEAR) on the antioxidant capacity of tilapia fillets over a period of 60 days. Different methodologies were used following the QUENCHER procedure, and the hydrophilic and lipophilic fractions of the oxygen radical absorbance capacity (ORAC FL) assay were analysed. The fatty acid composition was also evaluated, as high concentrations of linoleic and oleic acids were observed in the fillets, as well as satisfactory polyunsaturated fatty acids/saturated fatty acids (PUFA/SFA) ratios. The highest antioxidant capacities in 2,2-diphenyl-1-picrylhydrazyl (DPPH) and ferric reducing antioxidant power (FRAP) assays (1778.87 and 4892.77 µmol Trolox equivalent antioxidant capacity (TEAC) g-1, respectively) were found at 15 days, and these trials showed the highest correlation coefficient (R = 0.9388). The ORAC FL assay indicated that the hydrophilic fraction is the largest contributor to the total antioxidant capacity (TAC). Thus, the significant increase observed in antioxidant capacity makes supplementation with EEAR a potent tool in the elevation of the antioxidant capacity of tilapia fillets.

          Translated abstract

          Este trabalho avaliou o efeito da suplementação com extrato etanólico de resíduo de acerola (EEAR) na capacidade antioxidante de filés de tilápias no intervalo de 60 dias. Diferentes metodologias foram utilizadas seguindo procedimento QUENCHER e foram analisadas as frações hidrofílicas e lipofílicas do ensaio de capacidade de absorção de oxigênio radical (ORAC FL). A composição em ácidos graxos também foi avaliada, sendo observadas elevadas concentrações dos ácidos linoleico e oleico nos filés, assim como, razões ácidos graxos poli-insaturados/ácidos graxos saturados (PUFA/SFA) satisfatórias. Os maiores valores de capacidade antioxidante nos ensaios 2,2-difenil-1-picrilhidrazil (DPPH) e capacidade redutora de ferro (FRAP) (1778,87 e 4892,77 µmol capacidade antioxidante equivalente ao Trolox (TEAC) g-1, respectivamente) foram encontrados em 15 dias e esses ensaios apresentaram o maior valor de correlação (R = 0,9388). O ensaio ORAC FL indicou que a fração hidrofílica é a maior contribuinte na capacidade antioxidante total (TAC). Dessa forma, o significativo aumento observado na capacidade antioxidante torna a suplementação com EEAR uma potente ferramenta na elevação da capacidade antioxidante dos filés.

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          Most cited references34

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            The state of world fisheries and aquaculture 2008

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                Author and article information

                Contributors
                Role: ND
                Role: ND
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                Journal
                jbchs
                Journal of the Brazilian Chemical Society
                J. Braz. Chem. Soc.
                Sociedade Brasileira de Química (São Paulo )
                1678-4790
                July 2014
                : 25
                : 7
                : 1237-1245
                Affiliations
                [1 ] Universidade Estadual de Maringá Brazil
                [2 ] Universidade Estadual de Maringá Brazil
                Article
                S0103-50532014000700012
                10.5935/0103-5053.20140101
                0b2aa7be-3e9d-4028-8753-61db179373ed

                http://creativecommons.org/licenses/by/4.0/

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                SciELO Brazil

                Self URI (journal page): http://www.scielo.br/scielo.php?script=sci_serial&pid=0103-5053&lng=en
                Categories
                CHEMISTRY, MULTIDISCIPLINARY

                General chemistry
                tilapia,fatty acids,antioxidant capacity,QUENCHER procedure,ORAC FL assay
                General chemistry
                tilapia, fatty acids, antioxidant capacity, QUENCHER procedure, ORAC FL assay

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