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      Direct multiplexed measurement of gene expression with color-coded probe pairs

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          Abstract

          We describe a technology, the NanoString nCounter gene expression system, which captures and counts individual mRNA transcripts. Advantages over existing platforms include direct measurement of mRNA expression levels without enzymatic reactions or bias, sensitivity coupled with high multiplex capability, and digital readout. Experiments performed on 509 human genes yielded a replicate correlation coefficient of 0.999, a detection limit between 0.1 fM and 0.5 fM, and a linear dynamic range of over 500-fold. Comparison of the NanoString nCounter gene expression system with microarrays and TaqMan PCR demonstrated that the nCounter system is more sensitive than microarrays and similar in sensitivity to real-time PCR. Finally, a comparison of transcript levels for 21 genes across seven samples measured by the nCounter system and SYBR Green real-time PCR demonstrated similar patterns of gene expression at all transcript levels.

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          Author and article information

          Journal
          Nature Biotechnology
          Nat Biotechnol
          Springer Science and Business Media LLC
          1087-0156
          1546-1696
          March 2008
          February 17 2008
          March 2008
          : 26
          : 3
          : 317-325
          Article
          10.1038/nbt1385
          18278033
          0c042b30-394f-4c5c-a32d-69b1eb981447
          © 2008

          http://www.springer.com/tdm

          http://www.springer.com/tdm


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