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      Noninvasive glucose monitoring of the aqueous humor of the eye: Part I. Measurement of very small optical rotations.

      Diabetes Care
      Animals, Aqueous Humor, metabolism, Blood Glucose, analysis, Glucose, Humans, Hyperglycemia, blood, Hypoglycemia, In Vitro Techniques, Optical Rotation, Rabbits

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          Abstract

          We have described the concept of using the aqueous humor glucose as a measure of the blood glucose concentration, with a view to developing a noninvasive glucose monitor for diabetic individuals. We have conceived of a scleral lens that houses a light source, polarizers, other electro-optic units, and a light detector, and which measures the optical rotation of the aqueous humor continuously. We have built an optical bench mock-up of the glucose sensor and assessed the limits of its capabilities. We have described a physical method, employing the Faraday effect, that modulates the incident light and uses a compensator to introduce a feedback mechanism giving a null-point technique capable of measuring extremely small rotations with an accuracy of 0.4 s of arc. We have used this and have measured the optical rotations of glucose solutions from 0.02 to 0.1%, and have demonstrated linearity in both cases. Miniaturization of the technique is discussed.

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          Author and article information

          Journal
          7172992
          10.2337/diacare.5.3.254

          Chemistry
          Animals,Aqueous Humor,metabolism,Blood Glucose,analysis,Glucose,Humans,Hyperglycemia,blood,Hypoglycemia,In Vitro Techniques,Optical Rotation,Rabbits

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