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      Dicrocoelium dendriticum in cattle from Cypress Hills, Canada: humoral response and preliminary evaluation of an ELISA.

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      Veterinary parasitology

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          Abstract

          Apparent changes in the distribution of Dicrocoelium dendriticum in western Canada and the absence of other liver flukes in the area prompted an examination of the humoral responses in naturally infected cattle sharing pasture with heavily infected wild cervids. Western blots using sera from the infected cattle indicated that the cattle had antibodies to various fluke antigens, however the pattern of response varied among isotypes. Isotype analysis indicated that Dicrocoelium-infected cattle produced IgG1, and IgM antibodies, but not IgG2 antibodies. Subsequent comparison of the efficiency of coprological and serological approaches for diagnosis showed that while 43.5% of a separate group of 26 yearlings had eggs in their faeces, the immunoblots indicated that all had detectable antibodies. A group of cattle necropsied for confirmation of fluke status was used to evaluate humoral immune responses and to provide baseline information in the preliminary development of an indirect ELISA for detection of anti-fluke antibodies.

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          Author and article information

          Journal
          Vet. Parasitol.
          Veterinary parasitology
          1873-2550
          0304-4017
          Nov 24 2010
          : 174
          : 1-2
          Affiliations
          [1 ] Agriculture and Agri-Food Canada, Lethbridge Research Centre, Sustainable Production Systems, 5403 1st Ave. S., Lethbridge, AB, T1J 4B1, Canada. doug.colwell@agr.gc.ca
          Article
          S0304-4017(10)00429-2
          10.1016/j.vetpar.2010.08.004
          20817361
          0c599de6-9517-467b-8851-3ae5a1bd91b3
          Crown Copyright © 2010. Published by Elsevier B.V. All rights reserved.
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