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      Development of a one step real time RT-PCR assay to detect and quantify Dugbe virus


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          ► A one-step real time quantitative RT-PCR assay was developed to detect published Dugbe virus genomes of the Nairovirus genus. ► Primers and probes were designed to detect specific sequences on the most conserved regions of the S segment. ► The limit of detection was 10 copies per reaction improving of 3 log 10 FFU/mL the conventional RT-PCR. ► The specificity of the primers and probe was confirmed with the closely related Nairoviruses CCHFV and Hazara virus. ► 498 ticks collected in the Republic of Chad were screened: one sample was found positive.


          A one-step real time quantitative RT-PCR (qRT-PCR) assay was developed to detect all published Dugbe virus (DUGV) genomes of the Nairovirus genus. Primers and probes were designed to detect specific sequences on the most conserved regions of the S segment. The limit of detection of the assay was 10 copies per reaction which is an improvement of 3 log 10 FFU/mL over the sensitivity of conventional RT-PCR. The specificity of the primers and probe was confirmed with the closely related Nairoviruses CCHFV and Hazara virus, and on the non-related viruses Coronavirus and Influenza A virus. This qRT-PCR assay was used to screen nucleic acids extracted from 498 ticks collected in the Republic of Chad. One sample was found positive suggesting that DUGV is present in this part of the world. The molecular assay developed in this study is sensitive, specific and rapid and can be used for research and epidemiological studies.

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          Epidemiologic and clinical features of Crimean-Congo hemorrhagic fever in southern Africa.

          Following the diagnosis in 1981 of the first case of Crimean-Congo hemorrhagic fever (CCHF) in South Africa, an antibody survey was undertaken on cattle sera to determine the distribution of the virus and specific diagnostic tests were routinely applied to specimens from suspected cases of hemorrhagic fever to establish the medical significance of its presence. Antibody to CCHF virus was demonstrated by reversed passive hemagglutination-inhibition technique in 2,460/8,667 (28%) cattle sera and in 140/180 herds tested in South Africa, as well as in 347/763 (45%) cattle sera and in 32/34 (94%) herds tested in Zimbabwe. The antibody was found in all major cattle farming areas, but was of low prevalence along the southern coast where 2 of the 3 species of Hyalomma tick which occur in South Africa are absent. From February 1981 to January 1986, inclusive, 29 indigenous cases of CCHF were diagnosed in 16 outbreaks which arose in various locations throughout South Africa. A further 2 imported cases of CCHF arose in Zaire and Tanzania. The clinical features of infection conformed to the classical descriptions of CCHF in the Soviet Union. The fatal outcome in 11/31 cases indicates that the African disease is no less severe than that which occurs in Eurasia. It is inferred that the virus is widespread in all countries in Africa and Eurasia which lie within the limits of world distribution of ticks of the genus Hyalomma.
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            Investigation of tick-borne viruses as pathogens of humans in South Africa and evidence of Dugbe virus infection in a patient with prolonged thrombocytopenia.

            In the course of investigating suspected cases of viral haemorrhagic fever in South Africa patients were encountered who had been bitten by ticks, but who lacked evidence of infection with Crimean-Congo haemorrhagic fever (CCHF) virus or non-viral tick-borne agents. Cattle sera were tested by enzyme-linked immunoassay to determine whether tick-borne viruses other than CCHF occur in the country. The prevalence of antibody in cattle sera was 905/2116 (42.8%) for CCHF virus, 70/1358 (5.2%) for Dugbe, 21/1358 (1.5%) for louping ill, 6/450 (1.3%) for West Nile, 7/1358 (0.5%) for Nairobi sheep disease, 3/625 (0.5%) for Kadam and 2/450 (0.4%) for Chenuda. No reactions were recorded with Hazara, Bahig, Bhanja, Thogoto and Dhori viruses. The CCHF findings confirmed previous observations that the virus is widely prevalent within the distribution range of ticks of the genus Hyalomma, while antibody activity to Dugbe antigen was detected only within the distribution range of the tick Amblyomma hebraeum. Cross-reactivity for the nairoviruses, Hazara, Nairobi sheep disease and Dugbe, was detected in serum samples from 3/72 human patients with confirmed CCHF infection, and serum from 1/162 other patients reacted monospecifically with Dugbe antigen. The latter patient suffered from febrile illness with prolonged thrombocytopenia.
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              • Abstract: found
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              Is Open Access

              Tickborne Arbovirus Surveillance in Market Livestock, Nairobi, Kenya

              Viruses transmitted by blood-feeding arthropods (arboviruses) are responsible for some of the most serious emerging infectious disease problems facing the world today. Arthropodborne viruses constitute the largest biologic group of vertebrate viruses. Their considerable number and diversity suggest that arthropod vector transmission offers distinct survival benefits for the virus. Approximately 50% of arbovirus isolations from field-collected arthropods are from mosquitoes, and 25% are from ticks; however, this difference may represent a sampling bias, since many more mosquitoes are collected and tested for virus than ticks. To investigate the abundance of tickborne arboviruses in Kenya and the surrounding region, we collected and tested ticks infesting livestock driven to market at 2 major abattoirs in Nairobi, Kenya. These abattoirs receive the bulk of animals slaughtered for Nairobi and its environs, which is the largest livestock market in the country. Approximately 30% of animals slaughtered in these abattoirs come from within Kenya; the rest are from neighboring countries, including Ethiopia, Sudan, Somalia, and Tanzania. Among pastoral communities in this region, livestock are frequently maintained in enclosures close to human habitation, and small ruminants sometimes sleep inside houses overnight for security reasons. Such practices increase the potential for zoonotic virus transmission between animals and humans. Poor husbandry and grazing practices put great pressure on land resources, which results in the need to continuously move large numbers of animals, especially cattle, in search of pasture. In some parts of East Africa, these pastoral communities exist near wildlife parks, and wildlife and livestock sometimes mix, which allows transfer of ticks and possibly viruses between these animal groups. Additionally, livestock marketing practices allow movement of animals across borders in the region, which allows ticks and tickborne viruses to move between countries. Previous surveillance reports based on virus isolations or serologic studies in cattle from Kenya, the Central African Republic, and South Africa have identified tickborne arboviruses from the Bunyaviridae, Flaviviridae, Rhabdoviridae, Reoviridae, and Orthomyxoviridae ( 1 – 4 ). The genus Nairovirus, family Bunyaviridae, includes 37 named viruses that are principally tickborne ( 5 – 7 ). The most serious human pathogen among the tickborne viruses in the African region is Crimean-Congo hemorrhagic fever virus (CCHFV), a member of the Nairovirus genus that can cause fatal hemorrhagic disease ( 8 , 9 ). Outbreaks of Crimean-Congo hemorrhagic fever have occurred in People's Republic of China, South Africa, Pakistan, and Russia ( 10 – 12 ). The first reported human case of this disease in Kenya occurred recently at a farm that was heavily infested by ticks ( 13 ). Nairobi sheep disease virus (NSDV), also in the genus Nairovirus, causes fever, hemorrhagic gastroenteritis, and abortion in sheep and goats in East Africa ( 14 ). Epizootics of NSDV have been reported in parts of Africa where susceptible herds of sheep have been moved to NSDV-endemic areas, resulting in decimation of whole herds ( 14 ). Dugbe virus (DUGV), another member of the Nairovirus genus, has been repeatedly confirmed in tickborne virus surveys in Africa and causes febrile illness and thrombocytopenia in humans ( 2 ). Bhanja virus (BHAV), an unassigned member of the Bunyaviridae family, has also been isolated in this region ( 1 , 2 ). Other tickborne viruses present in Africa include Thogoto virus (THOV) (genus Thogotovirus, family Orthomyxoviridae), isolated in Kenya; Barur virus (family Rhabdoviridae), isolated in Somalia; and Kadam virus (KADV) (genus Flavivirus, family Flaviviridae) and Chenuda virus (genus Orbivirus, family Reoviridae), confirmed serologically in cattle in South Africa ( 1 , 2 , 4 ). Our study isolated and identified 6 previously known tickborne arboviruses, including DUGV, BHAV, THOV, Dhori virus (DHOV), KADV, and Barur virus. In addition, 2 viruses related to DUGV were isolated. An unexpected result of this study was the isolation of foot-and-mouth disease virus (FMDV) from tick pools. Materials and Methods Tick Collection and Processing Ticks were collected from the hides of flayed animals between September and November 1999 at the Njiru and Dagoretti abattoirs, on the outskirts of Nairobi, Kenya. Attached ticks were pulled off manually and placed in sterile plastic vials, which were loosely capped and transported to the laboratory. The origin of individual sampled animals could not be determined. All animals to be slaughtered for the day were put in 1 enclosure, irrespective of origin. In the laboratory, ticks were washed twice with sterile water to remove excess particulate contamination from animal hides, rinsed once with 70% ethanol, and then rinsed twice with minimum essential medium (MEM), with antimicrobial agents (100 U/mL penicillin, 100 μg/mL streptomycin, and 1 μL/mL amphotericin B). Ticks were identified by sex and species by using appropriate identification keys ( 15 , 16 ), transferred to sterile vials, and stored at –80°C until homogenized for virus isolation. Voucher specimens were prepared in ethanol, and identifications were reviewed at the International Centre of Insect Physiology and Ecology, Nairobi. Ticks were later thawed at room temperature, identifications were confirmed, and ticks were pooled into groups of 2 to 50, depending on the size of the ticks and according to species, collection dates, and sites. Each pool was homogenized by using 90-mesh alundum in a prechilled, sterile mortar and pestle with 1.6–2 mL ice cold bovine albumin (BA)-1 medium (1× medium 199 with Earle's salts, 1% BA, 100 U/mL penicillin, 100 μg/mL streptomycin, and 1 μL/mL amphotericin B). The homogenates were clarified by low-speed centrifugation at 1,500 rpm for 15 minutes at 4°C, and supernatants were aliquoted and stored at –80°C. In the case of Hyalomma species, the primary vectors of CCHFV, each pool was screened by reverse transcription–polymerase chain reaction (RT-PCR) for CCHFV before tissue culture injection was conducted (Table 1). Table 1 Reverse transcription–polymerase chain reaction primers used for arbovirus isolation, Nairobi, Kenya Virus target Target gene Primer designation Primer sequences* Crimean-Congo hemorrhagic fever N CCHF F2 TGGACACCTTCACAAACTC CCHF R3 GACAAATTCCCTGCACCA Dugbe N DG S1 TCTCAAAGACAAACGTGCCGCAG DG S5 TGCAACAACTGGATGTGTGA Nairobi sheep disease N NSD 12f GAATGGTCGAACGTGGAC NSD 16r TGCTGTCAGGACACCAGG Thogoto N THO NF CCTGCAGGGGCGGAAGTTATG THO NR AAAATCCTCGCAGTTGGCTATCA Flaviviruses NS5 FLAVI fu2 GCTGATGACACCGCCGGCTGGGACAC FLAVI cfd3 AGCATGTCTTCCGTGGTCATCCA Bunyamwera and California serogroups N BCS82C ATGACTGAGTTGGAGTTTCATGATGTCGC BCS332V TGTTCCTGTTGCCAGGAAAAT Rift Valley fever G2 RVF-M-727f GGAACCCCTGCATGAAAGAGA RVF-M-1565r CGATCCTGTGACGCAAACTC Dhori N DHO NF2 TGGTACCCTTTTCTTGCTTCACTCC DHO NR2 TGCTCTTCCTCGGCTCAAACACCA Babanki C/E3 BAB 1007f TGGCCATGGAAGGTAAGGTAAT BAB 1569r TATGGCGTTGAGCAGGGTATC Hazara N HAZ 803f CTGGTTGAGCTAGAGGGGAAAGACG HAZ 1304r GGGCGGCATCATCGGGACTG Coxsackie B4 Pol COX 6749f ACTTTGTGAGAGGGGGTATGC COX 7151r ACGTGGTATTGGGTGTTTTT Koutango NS5 KOU 176f TCAGGGAGGTGGGAGGTAAAC KOU 734r TCATGCCATCCAACAGAAGGT Saboya NS5 SAB 226f GCAGGCTGGGACACAAAGAT SAB 815r CTACAAGGGGCAATGATGGTTC Ndelle l3 NDE 655f GGGGTTTTCTGGCTAATGTCAC NDE 1238r GGGCCTGTCCAGTCTTTTTG Middleburg E2 MID 1939f TACATGCCCCGAAGGTGACT MID 2458r CGGGATGGTGTTCGGTAAAG *Primer sequences 5´–3´. Virus Isolation For virus isolation in cell culture, Vero cells were grown in 25-cm2 cell culture flasks to 80% confluency in MEM with 10% fetal bovine serum (FBS), 2% glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, and 1 μL/mL amphotericin B. Cells were rinsed with sterile saline, and 0.2 mL clarified tick homogenate was added followed by injection at 37°C for 45 minutes to allow virus adsorption. After incubation, cells were rinsed with saline and maintenance medium (MEM with Earle's salts, with 5% FBS, 2% glutamine, 100 U/mL penicillin, 100 μg/mL streptomycin, and 1 μL/mL amphotericin B) was added. Cells were incubated at 37°C and observed daily for signs of cytopathic effects (CPE). The pooled infection rate program (PooledInfRat, Centers for Disease Control and Prevention, Fort Collins, CO, USA; http://www.cdc.gov/ncidod/dvbid/westnile/software.htm) was used to compare virus infection rates in the tick species collected and processed in this study. Virus Identification Agents causing CPE in tissue culture were initially identified to virus group by using the indirect immunofluorescent antibody assay (IFA) on spot slides of infected Vero cells with polyvalent mouse hyperimmune ascitic fluids obtained from the National Institutes of Health Reference Reagents Program. Fluorescein isothiocyanate–conjugated goat-anti-mouse immunoglobulin G was the secondary antibody. RT-PCR was also used to identify most of the virus isolates obtained from tissue culture. RNA was extracted from cell culture supernatants with the QIAamp Viral RNA kit (Qiagen, Valencia, CA, USA) according to the manufacturer's recommended protocol. RT-PCR was performed with the Titan One Tube RT-PCR kit (Roche, Indianapolis, IN, USA) with primers mainly targeting the known African tickborne viruses (Table 1). References are available from the authors for previously published primers. All other primers were designed for this study to amplify a specific fragment from the virus listed and have not been tested for cross-reactivity with other related or unrelated viruses. RT-PCR was also performed on RNA extracted from uninfected Vero cells as a negative control. Amplified DNA fragments were visualized by electrophoresis on 0.8%–1.0% agarose gels. DNA fragments were extracted from gels with the QIAquick Gel Extraction Kit (Qiagen), and DNA was eluted in 20 μL 10 mmol/L Tris-HCl, pH 8.5, and stored at –20°C. RT-PCR fragments were sequenced with the CEQ DCTS Quick Start kit (Beckman Coulter, Inc., Fullerton, CA, USA) with listed primers and analyzed with a CEQ 8000 automated sequencer (Beckman Coulter, Inc.). Both strands of DNA were sequenced. Nucleic acid sequences were compared with the GenBank database by using the BLAST program (http://www.ncbi.nlm.nih.gov/BLAST). Additional methods used to identify selected isolates included complement fixation (CF) and hemagglutination-inhibition tests ( 17 ) and panviral microarray-based genotyping ( 18 ). Alignment of nucleic acid and deduced protein sequences was conducted by using the MegAlign program (Lasergene 6.1, DNASTAR, Inc., Madison, WI, USA); phylogenetic and molecular evolutionary analyses were conducted with MEGA version 3.0 (http://www.megasoftware.net). Results Tick Collection A total of 15,851 ticks were collected and processed in 1,071 pools for virus isolation. Species of ticks collected and their proportions in the collection are shown in Table 2. The predominant species collected was Rhipicephalus pulchellus (56%), followed by Amblyomma gemma, R. appendiculatus, and A. variegatum. Other species were sampled in smaller numbers. However, the calculated virus pooled infection rate was higher for A. gemma than for R. pulchellus (Table 2). The number of Hyalomma specimens collected was comparatively small (3%). These species are the primary vectors of CCHFV; this agent was not among the viruses isolated. Table 2 Tick species collected and their virus yield, Kenya* Species No. collected No. virus isolates Pooled infection rate Amblyomma variegatum 994 6 6.16 A. gemma 2,160 26 11.05 A. lepidum 963 4 4.24 A. coharens 4 0 0 Boophilus decoloratus 985 1 1.01 Hyalomma truncatum 270 0 0 H. albiparmatum 112 0 0 H. rufipes 96 0 0 H. dromedarii 1 0 0 Rhipicephalus appendiculatus 1,228 2 1.63 R. evertsi 146 0 0 R. pulchellus 8,892 17 2.48 Total 15,851 56 *DUGV, Dugbe virus; BHAV, Bhanja virus; THOV, Thogoto virus; DHOV, Dhori virus; KADV, Kadam virus; BARV, Barur virus, FMDV, foot-and-mouth disease virus. Virus Isolation and Identification A total of 56 virus isolates were obtained from 51 tick pools; 52 of the 56 viruses were identified (Table 3). Five pools contained 2 different viruses. All of the isolated viruses caused CPE in Vero cells. The observed onset of CPE was 4–10 days postinfection. In the initial identification by IFA, 6 isolates reacted positively with the Thogoto group–specific antiserum (polyvalent 4), 33 isolates reacted with the Congo group–specific antiserum, 1 isolate reacted with the flavivirus group–specific antiserum (group B), and 1 isolate reacted with antiserum that included specificity to DHOV (polyvalent 10). Table 3 Virus isolates obtained from ticks collected in Nairobi, Kenya Species Virus isolates DUGV DUGV-like BHAV THOV DHOV KADV BARV-like FMDV Unknown Total Amblyomma variegatum 6 0 0 0 0 0 0 0 0 6 A. gemma 16 5 0 4 0 0 0 0 1 26 A. lepidum 2 1 0 1 0 0 0 0 0 4 A. coharens 0 0 0 0 0 0 0 0 0 0 Boophilus decoloratus 0 0 1 0 0 0 0 0 0 1 Hyalomma. truncatum 0 0 0 0 0 0 0 0 0 0 H. albiparmatum 0 0 0 0 0 0 0 0 0 0 H. rufipes 0 0 0 0 0 0 0 0 0 0 H. dromedarii 0 0 0 0 0 0 0 0 0 0 Rhipicephalus appendiculatus 0 0 1 0 0 1 0 0 0 2 R. evertsi 0 0 0 0 0 0 0 0 0 0 R. pulchellus 2 1 0 1 1 0 6 3 3 17 Total 26 7 2 6 1 1 6 3 4 56 Forty-five virus isolates were identified by using RT-PCR and nucleic acid sequencing with primers specific to known tickborne viruses or by CF assay or microarray-based genotyping. The identified isolates included 26 DUGV, 6 THOV, 6 Barur virus, 3 FMDV, 2 BHAV, 1 DHOV, and 1 KADV. DUGV was isolated most frequently (46%). Most DUGV isolates were recovered from A. gemma (62%), whereas the most commonly sampled tick, R. pulchellus, yielded only 2 DUGV isolates (8%) (Table 3). Two of the virus isolates that were IFA-positive with Congo group antiserum were RT-PCR negative when primers specific for DUGV, CCHFV, Hazara virus, or BHAV, all of which were represented in the antiserum, were used. However, RT-PCR using NSDV nucleocapsid–specific primers and RNA extracted from these isolates produced 3 major bands, including one ≈880 bp in size; the expected band size for the NSDV-specific fragment was 887 bp. The 880-bp fragment was sequenced, and an alignment of 513 nt (nt) of this sequence with nucleocapsid sequences from DUGV, CCHFV, and NSDV showed 71%, 58%, and 60% homology, respectively, which suggests that these isolates were most closely related to DUGV. Alignment of the sequences from the 2 isolates showed them to be 95% homolgous. Specific primers were designed for this DUGV-like virus from sequence of the fragment described above. RT-PCR conducted with these primers produced bands of correct size and sequence with RNA from the DUGV-like virus isolates, while RT-PCR results using these primers with RNA from DUGV and BHAV were negative. RT-PCR and sequencing of all of the virus isolates using the primers designed from the DUGV-like virus sequence showed 4 additional isolates of this DUGV-like virus; 2 were from pools that also contained DUGV. Sequence homology between all 6 DUGV-like isolates was 95%–100%, based on a 508-nt alignment of the S segment of the virus RNA. Of the 6 isolates of this virus, 5 (83%) were from A. gemma pools, and 1 (17%) was from a pool of R. pulchellus. One additional isolate, obtained from a pool of A. lepidum, was identified as being DUGV-like by CF test. RT-PCR conducted on RNA extracted from this isolate with the primers designed for the DUGV-like virus described above produced a weak band. Sequence of this product was ≈80% homologous to the other isolates of DUGV-like virus and 70%, 63%, 57%, and 55% homologous to DUGV, NSDV, CCHFV, and Hazara virus, respectively, which suggests that it may be a different DUGV-like isolate. Six isolates of THOV were obtained: 4 from pools of A. gemma, l from A. lepidum, and 1 from R. pulchellus. Since the THOV genome is segmented, a portion of each of the 6 genome segments from each isolate was sequenced and compared with available sequence from other THOV isolates to determine if reassortment of virus genome segments had occurred. No evidence was found for reassortment of virus segments. Phylogenetic analysis showed that the THOV isolates were most closely related to other African isolates from Uganda (1996), Kenya (1960), and Nigeria (1969) (data not shown). The single isolate of DHOV, another member of the tickborne orthomyxovirus group, was obtained from a pool of R. pulchellus. A single isolate of KADV, the only African member of the tickborne flavivirus group, was recovered from a pool of R. appendiculatus. Six isolates were found by CF test to be indistinguishable from Barur virus, a rhabdovirus. Further characterization of these isolates was not conducted. Three isolates of FMDV were identified by using panviral microarray-based technology. RNA isolated from viral culture of 1 isolate was reverse transcribed, randomly amplified, and hybridized to panviral DNA microarrays as described ( 19 ). Analysis of the hybridization patterns showed extensive hybridization to oligonucleotides derived from FMDV. Based on this result, PCR primers were designed from conserved regions of FMDV to confirm the identity of the virus. A PCR product of ≈600 bp was generated; it possessed 97% nucleotide identity to FMDV serotype SAT3. Two other isolates were identical by CF test. Four isolates remained unidentified. Three of these were recovered from R. pulchellus and 1 from A. gemma. All of these isolates failed to react with the hyperimmune ascites grouping fluids used for the IFA identification procedure and produced negative results in RT-PCR tests when primers specific to known African tickborne viruses were used. Discussion In this study, A. gemma ticks were incriminated for the first time as key vectors or reservoirs of tickborne viruses in the East African region; 46% of our virus isolates were obtained from this species. Distribution limits of ticks are variable and are influenced by several factors, including climate, vegetation, host density, host susceptibility, and host grazing habits. During previous studies conducted at the Lake Victoria basin in Kenya ( 1 ), A. gemma was not collected, most likely because this species is limited to more arid zones. A. gemma is found only in the dry zones of bushwillows (Combretum) and shrub steppe and is much more restricted than A. lepidum to very dry areas. DUGV and DUGV-like viruses were the most frequently isolated viruses in this study (33/56, 59%), and 64% of these isolates were from pools of A. gemma. Four of the 6 THOV isolates were obtained from pools of this species as well. Our results suggest that viruses are being actively transmitted in the drier parts of East Africa where A. gemma is more common. The pastoral regions, which supply many of the animals slaughtered at abattoirs in Nairobi, are predominantly dry and therefore likely to harbor this tick species in abundance. The proportion of R. appendiculatus collected in this study was small when one considers the distribution of this tick in Kenya and its importance as a pest, a finding that suggests that most sampled cattle came from climatic zones where this species is not abundant. In Tanzania and Kenya, R. appendiculatus is most abundant in areas receiving >1,000 mm mean annual rainfall. It is absent from xerophytic and dry thicket zones with overgrazed pastures and little grass cover ( 15 ). NSDV is mainly transmitted by R. appendiculatus, and the virus is found only in areas where this species is abundant ( 14 ); therefore, the relatively low numbers of this species collected may explain why NSDV was not isolated in this study. Pools of R. appendiculatus, however, did yield single isolates of BHAV and KADV. BHAV has been isolated previously in Kenya and Nigeria ( 1 , 20 ). The medical implications of this virus for humans and animals in this region have not been determined, although the virus has been associated with human infection and illness in eastern Europe and West Africa ( 21 – 23 ). KADV is the only known African tickborne flavivirus. The virus was first isolated from R. pravus ticks taken from a cow in Uganda ( 24 , 25 ) and later in Kenya from A. variegatum and R. pulchellus ( 14 ). Although KADV pathogenicity is not evident in humans, antibodies against KADV were detected in human sera during a serosurvey in Uganda ( 26 ). DUGV is commonly isolated in surveillance studies conducted in Africa ( 1 , 2 , 27 , 28 ), and it appears to be endemic in most of the drier parts of the continent. The implications of DUGV for human health have not been evaluated in Kenya, although reports from other regions in Africa suggest that human infection and illness caused by DUGV infection occur ( 2 , 22 , 27 ). Johnson et al. ( 1 ), in an earlier study conducted around Lake Victoria, recovered more DUGV isolates than any other virus and observed that more tick pools from dry scrub land (away from the lake) were infected with DUGV than pools from the swamp edge. These researchers also observed that 12 of the 39 DUGV isolates recovered varied in their behavior in cell culture and in suckling mice, which suggests that some of the DUGV strains isolated were different. In our study, in addition to the 26 isolates of DUGV, we identified 2 DUGV-like viruses (6 isolates of one, 1 isolate of another), which were found to differ significantly in S segment nucleotide sequence from previously published DUGV sequences. Further investigation of these isolates is necessary to determine their relatedness to DUGV. THOV was first isolated in Kenya from Rhipicephalus species and Boophilus decoloratus in the 1930s ( 29 ) and has been isolated repeatedly from various tick species in Kenya, West Africa, Europe, and Asia ( 30 ). Two THOV infections have been reported in humans, with 1 fatality ( 22 ). In our survey, THOV was isolated from pools of A. gemma ( 4 ), A. lepidum ( 1 ), and R. pulchellus ( 1 ). DHOV, also a member of the Thogotovirus genus in the Orthomyxovirdae family, has been previously isolated in Europe, Asia, and the Middle East ( 31 – 34 ). Human DHOV infection has been evidenced by serologic survey results and human illness ( 23 , 34 , 35 ). We report here the first isolation of DHOV in East Africa. This finding suggests a southward spread of the virus that is supported by the presence of competent tick vectors in the region and demonstrates the potential for other tickborne viruses circulating in Europe and Asia to spread to the African continent. Such spread would have adverse consequences for large, immunologically naive populations whose pastoral practices provide for closer human-animal contact. An unexpected finding in this study was the isolation of FMDV from 3 pools of R. pulchellus. FMDV is endemic in many parts of Africa; however, it has not previously been identified in association with tick surveillance or transmission studies ( 36 ). This finding does not constitute evidence that FMDV replicates in or can be transmitted by ticks; in fact, previous reports indicate that the virus is not transmissible by Rhipicephalus ticks or blood-feeding flies ( 37 , 38 ). However, the virus has been demonstrated to persist in ticks for up to several days after feeding on an infected animal ( 37 ). The ticks in our study were not held for any length of time to allow for blood in the ticks to be digested before processing for virus isolation. Therefore, FMDV may have been present in undigested blood in >1 ticks in each pool. FMDV is present in the blood of an infected animal, skin lesions, and skin areas that do not contain lesions ( 39 ). The virus persists in skin up to 4 days beyond the period of viremia and for extended periods in preserved hides ( 40 ). Therefore, mouth parts of ticks feeding on FMDV-infected cattle might have become contaminated with the virus, which was then not sufficiently exposed to the external rinsing procedures to which the ticks were subjected before processing. Further investigation is necessary to clarify the mechanism of these FMDV isolations and the implications of these findings. Our study illustrates the potential for tickborne dissemination of endemic and emergent viruses, some of which are human pathogens, among livestock as well as the potential for transmission of these pathogens to humans. Regular surveillance is warranted to monitor the presence and spread of these and other viruses facilitated through livestock rearing, marketing, and movement in Africa.

                Author and article information

                J Virol Methods
                J. Virol. Methods
                Journal of Virological Methods
                Elsevier B.V.
                14 June 2011
                September 2011
                14 June 2011
                : 176
                : 1
                : 74-77
                [a ]Emerging Pathogens Laboratory, Fondation Mérieux, 21 Avenue Tony Garnier, 69007 Lyon, France
                [b ]Unité de Virologie, Institut de Recherche Biomédicale des Armées-Antenne de la Tronche, 27 Avenue des Maquis du Grésivaudan, 38000 La Tronche, France
                [c ]Groupe de Travail en Epidémiologie Animale du Service de Santé des Armées, Marseille, France
                [d ]Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes UMR 6236, Marseille, France
                Author notes
                [* ]Corresponding author at: Emerging Pathogenes Laboratory, Fondation Mérieux, 21 Avenue Tony Garnier, 69007 Lyon, France. Tel.: +33 4 37 28 24 20; fax: +33 4 37 28 24 11. cpeyrefitte2000@ 123456yahoo.fr
                Copyright © 2011 Elsevier B.V. All rights reserved.

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                : 17 February 2011
                : 1 June 2011
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                Microbiology & Virology
                nairovirus,dugbe virus,one step real-time rt-pcr,screening tool,tick vector,epidemiology


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