8
views
0
recommends
+1 Recommend
0 collections
    0
    shares
      • Record: found
      • Abstract: found
      • Article: not found

      Cloning of expansin genes in ramie (Boehmeria nivea L.) based on universal fast walking.

      Read this article at

      ScienceOpenPublisherPubMed
      Bookmark
          There is no author summary for this article yet. Authors can add summaries to their articles on ScienceOpen to make them more accessible to a non-specialist audience.

          Abstract

          Gene cloning is the first step to study the expression profiles and functions of a particular gene; considerable cloning methods have been developed. Expansin, thought to involve in the cell-wall modification events, was not cloned in ramie (Boehmeria nivea L.), which is one of the most important bast fiber crops with little conducted molecular research, especially on its fiber development. Studying the expansin gene family will uncover its possible relationship with ramie fiber development and other growth events. As a result, five expansin genes were cloned with full-length and their sequence information was investigated. Additionally, the phylogenetic analysis was conducted, which suggested that the cloned genes belong to the α-subfamily, and these genes expressed differently during ramie fiber developmental process. In this study, we aimed to apply a strategy for cloning novel full-length genes from genomic DNA of ramie, based on using degenerate primers, touchdown polymerase chain reaction and universal fast walking protocols. By cloning five full-length expansin genes, we believe the polymerase chain reaction-based gene cloning strategy could be applied to general gene studies in ramie and other crops.

          Related collections

          Author and article information

          Journal
          Gene
          Gene
          Elsevier BV
          1879-0038
          0378-1119
          Sep 10 2015
          : 569
          : 1
          Affiliations
          [1 ] MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, #1 Shizishan Street, Hongshan District, Wuhan 430070, Hubei Province, China. Electronic address: lqlcj@126.com.
          [2 ] MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, #1 Shizishan Street, Hongshan District, Wuhan 430070, Hubei Province, China. Electronic address: dailunjin@gmail.com.
          [3 ] MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, #1 Shizishan Street, Hongshan District, Wuhan 430070, Hubei Province, China. Electronic address: wangbo@mail.hzau.edu.cn.
          [4 ] MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, #1 Shizishan Street, Hongshan District, Wuhan 430070, Hubei Province, China. Electronic address: liulijun@mail.hzau.edu.cn.
          [5 ] MOA Key Laboratory of Crop Ecophysiology and Farming System in the Middle Reaches of the Yangtze River, College of Plant Science and Technology, Huazhong Agricultural University, #1 Shizishan Street, Hongshan District, Wuhan 430070, Hubei Province, China. Electronic address: pdxiang@mail.hzau.edu.cn.
          Article
          S0378-1119(14)01291-8
          10.1016/j.gene.2014.11.029
          25481635
          0d3fe767-f98e-46d0-b18f-b69c6af7d1c4
          History

          Degenerate primer,Touchdown PCR,Ramie,Gene cloning,Expansin
          Degenerate primer, Touchdown PCR, Ramie, Gene cloning, Expansin

          Comments

          Comment on this article