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      Scanning Electron Microscopy of Aortic Endothelial Cell Boundaries After Staining with Silver Nitrate

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      Journal of Vascular Research

      S. Karger AG

      Aorta, Thoracic, Endothelium, Microscopy, Scanning electron, Silver nitrate, Staining

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          Abstract

          Scanning electron microscopy of the rabbit thoracic aorta following staining with silver nitrate showed endothelial cell borders to be marked by zigzag lines outlining individual endothelial cells as oblong rhombic fields oriented with the long axis parallel to the aortic axis. The layer of endothelial cells coated an underlying layer of coarse longitudinal folds, probably foldings of the underlying elastic membranes. The individual endothelial cells were inconspicuous in uncoated specimens. Double coating with coal and gold revealed a grainy porous mass in the silver lines and a flat surface of endothelial cells studded with small round elevations. Comparative studies with transmission light microscopy and transmission electron microscopy indicated silver deposits in the endothelial cell boundaries. Fine dustlike silver sediments were also observed on the surface of endothelial cells and in the layers next to the intima.

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          Author and article information

          Journal
          JVR
          J Vasc Res
          10.1159/issn.1018-1172
          Journal of Vascular Research
          S. Karger AG
          1018-1172
          1423-0135
          1970
          1970
          18 September 2008
          : 7
          : 6
          : 365-373
          Affiliations
          Anatomy Department A, University of Copenhagen, Copenhagen, and Department of Anatomy, University of Odense, Odense
          Article
          157852 Angiologica 1970;7:365–373
          10.1159/000157852
          © 1970 S. Karger AG, Basel

          Copyright: All rights reserved. No part of this publication may be translated into other languages, reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, microcopying, or by any information storage and retrieval system, without permission in writing from the publisher. Drug Dosage: The authors and the publisher have exerted every effort to ensure that drug selection and dosage set forth in this text are in accord with current recommendations and practice at the time of publication. However, in view of ongoing research, changes in government regulations, and the constant flow of information relating to drug therapy and drug reactions, the reader is urged to check the package insert for each drug for any changes in indications and dosage and for added warnings and precautions. This is particularly important when the recommended agent is a new and/or infrequently employed drug. Disclaimer: The statements, opinions and data contained in this publication are solely those of the individual authors and contributors and not of the publishers and the editor(s). The appearance of advertisements or/and product references in the publication is not a warranty, endorsement, or approval of the products or services advertised or of their effectiveness, quality or safety. The publisher and the editor(s) disclaim responsibility for any injury to persons or property resulting from any ideas, methods, instructions or products referred to in the content or advertisements.

          Page count
          Pages: 9
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          Paper

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