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      The Opposing Actions of Arabidopsis CHROMOSOME TRANSMISSION FIDELITY7 and WINGS APART-LIKE1 and 2 Differ in Mitotic and Meiotic Cells

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          Abstract

          Detailed mutant analysis uncovers epistatic interactions between Arabidopsis CTF7 and WAPL, which help regulate sister chromatid cohesion during meiosis and mitosis.

          Abstract

          Sister chromatid cohesion, which is mediated by the cohesin complex, is essential for the proper segregation of chromosomes during mitosis and meiosis. Stable binding of cohesin with chromosomes is regulated in part by the opposing actions of CTF7 (CHROMOSOME TRANSMISSION FIDELITY7) and WAPL (WINGS APART-LIKE). In this study, we characterized the interaction between Arabidopsis thaliana CTF7 and WAPL by conducting a detailed analysis of wapl1-1 wapl2 ctf7 plants. ctf7 plants exhibit major defects in vegetative growth and development and are completely sterile. Inactivation of WAPL restores normal growth, mitosis, and some fertility to ctf7 plants. This shows that the CTF7/WAPL cohesin system is not essential for mitosis in vegetative cells and suggests that plants may contain a second mechanism to regulate mitotic cohesin. WAPL inactivation restores cohesin binding and suppresses ctf7-associated meiotic cohesion defects, demonstrating that WAPL and CTF7 function as antagonists to regulate meiotic sister chromatid cohesion. The ctf7 mutation only had a minor effect on wapl-associated defects in chromosome condensation and centromere association. These results demonstrate that WAPL has additional roles that are independent of its role in regulating chromatin-bound cohesin.

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          Author and article information

          Journal
          Plant Cell
          Plant Cell
          plantcell
          aspb
          The Plant Cell
          American Society of Plant Biologists
          1040-4651
          1532-298X
          February 2016
          26 January 2016
          : 28
          : 2
          : 521-536
          Affiliations
          [a ]Department of Chemistry and Biochemistry, Miami University, Oxford, Ohio 45056
          [b ]University of Costa Rica, Fabio Baudrit Agricultural Research Station, La Garita de Alajuela, 20102, Costa Rica
          [c ]Institute of Plant and Microbial Biology, Academia Sinica, Nankang, Taipei 11529, Taiwan
          [d ]Biotechnology Center, National Chung-Hsing University, Taichung 402, Taiwan
          Author notes
          [1 ]Address correspondence to makaroca@ 123456miamioh.edu .

          The author responsible for distribution of materials integral to the findings presented in this article in accordance with the policy described in the Instructions for Authors ( www.plantcell.org) is: Christopher A. Makaroff ( makaroca@ 123456miamioh.edu ).

          Author information
          http://orcid.org/0000-0001-9729-9608
          http://orcid.org/0000-0003-1729-0561
          http://orcid.org/0000-0001-8830-4663
          http://orcid.org/0000-0003-3459-1331
          http://orcid.org/0000-0001-6237-1868
          Article
          PMC4790872 PMC4790872 4790872 TPC201500781RAR1
          10.1105/tpc.15.00781
          4790872
          26813623
          0d983a5e-4247-4f40-b534-c1cfc3739f0c
          © 2016 American Society of Plant Biologists. All rights reserved.
          History
          : 08 September 2015
          : 06 January 2016
          : 21 January 2016
          Page count
          Pages: 16
          Categories
          Research Articles
          Custom metadata
          v1

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